LOCUS       sequence-134802-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-HA-Sestrin2
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tccaactgca cctcggttct atcgattgaa ttcccgaacc gacagtcggt ctcttcacca
       61 aggccattcg cgccaccatg ggctacccct atgatgtgcc tgactacgca gatctcaatg
      121 gtggtggtgg tgggtcgacg atgatcgtgg cggactccga gtgccgcgca gagctcaagg
      181 actacctgcg gttcgccccg ggcggcgtcg gcgactcggg ccccggagag gagcagaggg
      241 agagccgggc tcggcgaggc cctcgagggc ccagcgcctt catccccgtg gaggaggtcc
      301 ttcgggaggg ggctgagagc ctcgagcagc acctggggct ggaggcactg atgtcctctg
      361 ggcgagtaga caacctggca gtggtgatgg gcctgcaccc tgactacttt accagcttct
      421 ggcgcctgca ctacctgctg ctgcacacgg atggtccctt ggccagctcc tggcgccact
      481 acattgccat catggctgcc gcccgccatc agtgttctta cctggtaggc tcccacatgg
      541 ccgagtttct gcagactggt ggtgaccctg agtggctgct gggcctccac cgggcccccg
      601 agaagctgcg caaactcagc gagatcaaca agttgctggc gcatcggcca tggctcatca
      661 ccaaggaaca catccaggcc ttgctgaaga ccggcgagca cacttggtcc ctggccgagc
      721 tcattcaggc tctggtcctg ctcacccact gccactcgct ctcctccttc gtgtttggct
      781 gtggcatcct ccctgagggg gatgcagatg gcagccctgc cccccaggca cctacacccc
      841 ctagtgaaca gagcagcccc ccaagcaggg acccgttgaa caactctggg ggctttgagt
      901 ctgcccgcga cgt
//
