LOCUS       sequence-134804-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin1 isoform1
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..85
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggtgtcact cccaggtcca actgcacctc ggttctatcg attgaattca ccatggaaat
       61 ggactacaag gatgacgatg acaagggtgg tggtggtggt gcgtcgacga tggccgaagg
      121 agaaaatgaa gtgcggtggg atgggctgtg ctcaagggac tcaacaacaa gggagactgc
      181 tctggaaaat atcaggcaga caatcctgag aaaaactgaa tatctgagga gtgtgaagga
      241 gactccccac aggcctagtg acgggctgtc aaacaccgag agctccgatg gactgaataa
      301 gctgctggct catctgctga tgctgtcaaa gaggtgccct ttcaaagatg tgcgcgaaaa
      361 gagcgagttt atcctgaaat ccattcagga gctgggcatc aggattccac gccccctggg
      421 acagggacca agcaggttta tccccgaaaa ggagattctg caagtgggat ccgaggacgc
      481 ccagatgcac gctctgttcg cagattcttt tgccgctctg ggccgcctgg acaacatcac
      541 cctggtcatg gtcttccacc cacagtatct ggagtctttt ctgaaaaccc agcattacct
      601 gctgcagatg gacggacctc tgccactgca ctaccgccat tatatcggca ttatggcagc
      661 cgctcgacac cagtgttcct acctggtgaa cctgcacgtc aatgacttcc tgcatgtggg
      721 cggggatcct aagtggctga acgggctgga aaatgcacca cagaaactgc agaacctggg
      781 agagctgaat aaggtgctgg cacaccgacc atggctgatc acaaaagaac atattgaggg
      841 gctgctgaag gctgaggaac actcatggag cctggccgaa ctggtgcatg ctgtggtcct
      901 gctgacccac tatcattccc tggcatcttt cacatttggc tgcgggatct ccccagagat
      961 tcactgtgat ggaggccata cc
//
