LOCUS       sequence-134805-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin1 isoform1
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgcataaca gttgggccat ggcggccaag ctggggatcc ttacttactt agcggccgcg
       61 tcaagtcata taccgagtaa tagccctcag agcatacagc agttcagcct gcattcgtgc
      121 ctcaatcagc agcagattga catgcacttt ttcgctgtgc ttaaactgcc gccagaatga
      181 gtcgtacatt cgtttggtga ccttctcagg ggtacagacc acagttttga tatacacctt
      241 gaaacttctg tccagcagct gattaatttc cccataatcg taatcgtcgt accggatgcc
      301 aaacatgcag tgaatatagt tccagatggc tctccgcagc atagaagtat ccacgtcctt
      361 atgcattgcc attgtattat aggtcaggtt gtaagcaatg tggaatttct catcgatcag
      421 ctgccccacg tcagggtaca ggcggttcac cagggaatag ccatgatctt cccaacagta
      481 gtcctggacc ctgaatgtgg gcacatgcat gccgtgtctg gaaaaatcct tatatccgta
      541 gctagtgtcc tcgaagtgcc gtgagacggc tcgtgcgggt gtcacctctt catcgtcact
      601 agaaaacacg aacatgcttt ctctcttctc gatttcgaac cgactggcca tctcttcctg
      661 agatgcctct tcctcgtctc ggcactcctg cagctggcgc attttttcca tcagagcctc
      721 cacttcaaag aagctatctg agacactcac attctcggca ctgttgacgg gcatttcgtc
      781 cacagagtga ttgccgtttg taatatcaca gatgcagtaa ttagacacgg aagggggtct
      841 aaaggtatgg cctccatcac agtgaatctc tggggagatc ccgcagccaa atgtgaaaga
      901 tgccagggaa tgatagtggg tcagcaggac cacagcatgc accagttcgg ccaggctcca
      961 tgagtgttcc tcagccttca gcagcccctc aatatgttc
//
