LOCUS       sequence-134806-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin2
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccaactgcac ctcggttcta tcgattgaat tcaccatgga aatggactac aaggatgacg
       61 atgacaaggg tggtggtggt ggtgcgtcga cgatgatcgt ggcggactcc gagtgccgcg
      121 cagagctcaa ggactacctg cggttcgccc cgggcggcgt cggcgactcg ggccccggag
      181 aggagcagag ggagagccgg gctcggcgag gccctcgagg gcccagcgcc ttcatccccg
      241 tggaggaggt ccttcgggag ggggctgaga gcctcgagca gcacctgggg ctggaggcac
      301 tgatgtcctc tgggcgagta gacaacctgg cagtggtgat gggcctgcac cctgactact
      361 ttaccagctt ctggcgcctg cactacctgc tgctgcacac ggatggtccc ttggccagct
      421 cctggcgcca ctacattgcc atcatggctg ccgcccgcca tcagtgttct tacctggtag
      481 gctcccacat ggccgagttt ctgcagactg gtggtgaccc tgagtggctg ctgggcctcc
      541 accgggcccc cgagaagctg cgcaaactca gcgagatcaa caagttgctg gcgcatcggc
      601 catggctcat caccaaggaa cacatccagg ccttgctgaa gaccggcgag cacacttggt
      661 ccctggccga gctcattcag gctctggtcc tgctcaccca ctgccactcg ctctcctcct
      721 tcgtgtttgg ctgtggcatc ctccctgagg gggatgcaga tggcagccct gccccccagg
      781 cacctacacc ccctagtgaa cagagcagcc ccccaagcag ggacccgttg aacaactctg
      841 ggggctttga gtctgcccgc gacgtgg
//
