LOCUS       sequence-134808-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin3
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgcataac agttgggcca tggcggccaa gctggggatc cttacttact tagcggccgc
       61 gttaggtcag atggcgagta atagccctca gagcatacag cagttcagcc tgcattcttg
      121 cttccatcag cagcaggttg acatgcactt tctcagagtg cttaaactgc cgccaatagg
      181 agtcgtacat ccttttggta gttctctcgg gataacaagt gactgtcttg atgtacacct
      241 tcagagatct ttccagcagc tgattcactt cgccataatc gtaatcgtca taccggattc
      301 caaacatgca gtggacgtag ttgaacagtg cgcgcctcag cattgtggta tccacgtctt
      361 catgagtagc cattgtatta taggtcaggt tgtagaccat gcggaacttc tcatccagca
      421 ggtgtccaat gtcagaatac agcctattca ccagggaaaa cccatggttc tcccaagtgt
      481 agtcctgggc gcggaatgtg ggcaggtgtt cctcgcctct ccgtgcaaag tcttcatatc
      541 cgaaccctgg atcctcgatg tacctactca cgtcagaggt aatgatcata tcgtcttcaa
      601 agtcagaatg aggaaaggag tggaaagtat cgccactgac cacaaacaga gactctttct
      661 tttctttctc gaatcgggtg gacatttcct cctggctagc ttcctcgtct tcgcgttcct
      721 cctgcagccg cttcattcgc tccatcaggg cttccagctc ggacaggcta tccacaatcc
      781 cgaagtttga gccactcaga gatgcattct cgatattgtt atcgttagcc aggtcacaga
      841 cgcaaaaatt gttcacgcta atcaggcgga agccatttga gatttcagga tccctctcgg
      901 ggttgatgcc tgatccaaac acgaaactag ccagggcatg gtagtgggcc agcaggacca
      961 ctgcgt
//
