LOCUS       sequence-134812-        6411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human IL23p19 in mammalian cells (V5 tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ramnath D, Tunny K, Hohenhaus DM, Pitts CM, Bergot AS, Hogarth PM, 
            Hamilton JA, Kapetanovic R, Sturm RA, Scholz GM, Sweet MJ
  TITLE     TLR3 drives IRF6-dependent IL-23p19 expression and p19/EBI3 
            heterodimer formation in keratinocytes.
  JOURNAL   Immunol Cell Biol. 2015 Oct;93(9):771-9. doi: 10.1038/icb.2015.77. 
            Epub 2015 Aug 25.
  PUBMED    26303210
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/icb.2015.77"; journalName: "Immunol Cell Biol"; date: 
            "2015-10"; volume: "93"; issue: "9"; pages: "771-9"
COMMENT     Sequence Label: hIL23p19 pEF6-V5 (MJS_004)
FEATURES             Location/Qualifiers
     source          1..6411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             637..678
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             688..705
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(728..745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    734..958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1004..1432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1091..1110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1301..1322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1441..1461)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1446..1776
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1627..1762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1689..1708
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1824..1871
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1890..2288
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2446..2567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2483..2502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2537..2556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2616..2632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2616..2632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2629..2651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2640..2656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2664..2670,2671..2688,2689..2694))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2709..2730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2847..2864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3018..3606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3098..3117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3777..4568,4569..4637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4400..4419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4638..4742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4817..4836)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5126..6304
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5357..6295
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6252..6272
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6321..6340
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6321..6339
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagatgctgg ggagcagagc tgtaatgctg ctgttgctgc tgccctggac agctcagggc
       61 agagctgtgc ctgggggcag cagccctgcc tggactcagt gccagcagct ttcacagaag
      121 ctctgcacac tggcctggag tgcacatcca ctagtgggac acatggatct aagagaagag
      181 ggagatgaag agactacaaa tgatgttccc catatccagt gtggagatgg ctgtgacccc
      241 caaggactca gggacaacag tcagttctgc ttgcaaagga tccaccaggg tctgattttt
      301 tatgagaagc tgctaggatc ggatattttc acaggggagc cttctctgct ccctgatagc
      361 cctgtgggcc agcttcatgc ctccctactg ggcctcagcc aactcctgca gcctgagggt
      421 caccactggg agactcagca gattccaagc ctcagtccca gccagccatg gcagcgtctc
      481 cttctccgct tcaaaatcct tcgcagcctc caggcctttg tggctgtagc cgcccgggtc
      541 tttgcccatg gagcagcaac cctgagtccc aagggcaatt ctgcagatat ccagcacagt
      601 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
      661 ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc
      721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
      901 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
      961 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     1021 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1081 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1141 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
     1201 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1261 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1321 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
     1381 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     1441 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     1501 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     1561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     1621 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     1681 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     1741 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     1801 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     1861 tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca
     1921 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     1981 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     2041 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     2101 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     2161 gacggtgtcg acaggtgctt ctcgatctgc atcctgggat caaagcgata gtgaaggaca
     2221 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     2281 agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     2341 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     2401 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     2461 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2521 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     2581 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2641 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2701 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2761 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2821 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2881 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2941 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3001 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3061 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3121 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3181 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3421 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3901 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3961 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4021 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4081 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4141 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4201 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4261 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4321 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4381 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4441 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4501 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4561 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4621 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4741 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     4801 tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     4861 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     4921 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     4981 gcgatgtacg ggccagatat acgcgttgac attgattatt gactaggctt ttgcaaaaag
     5041 ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
     5101 aggtcttgaa aggagtgcct cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
     5161 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     5221 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5281 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5341 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     5401 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     5461 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     5521 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     5581 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     5641 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     5701 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     5761 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     5821 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     5881 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     5941 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     6001 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     6061 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     6121 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     6181 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     6241 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     6301 gtgaggaatt agcttggtac taatacgact cactataggg agacccaagc tggctaggta
     6361 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattgccct t
//
