LOCUS       sequence-134845-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 
            2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: 2PH-PLCd-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..85)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gctcgctcac ggtaccgctt ccaggagcag gaccggggtt ttcttccacg tctcctgctt
       61 gctttaacag agagaagttc gtggctccgg atcctgcctc ggcgaatccc agttcagaca
      121 gtttcaggtg tgcctcagca tagtcggcga agaacacatc ctcgtcggct gcatactttt
      181 cgaccagagg cctgaacacg ctatctgtca gcagggcttt gtcagagggc agctgcagca
      241 gtccatcctt ctcgccggtc agcagttcag taaagtagga attgtcgaag atcagagggt
      301 tgcttgtcca gggtccctcg aagcctgagc gttccttatg agcggcgcca atggtgtgcc
      361 ctccactcag ggccacgata tcctggtcag acagccccat tgcttttccg aacacgtccc
      421 gcagatggtc gctgcccttg gtagcatctg gcagtctccc ctctggaggg ggttcaggtt
      481 tgtcctccct tcctgggtgg aaagggacct tggggccgcc ggtaatctcc acagcgacca
      541 cgccggccag ctggtagaag tctgcgtagg agacaatggg gaactgttct ttgattggct
      601 ccagcagtct cacagcaatg tccaggccat tgttggcccc atgtgccagt tcagcctggt
      661 gcttgatagt cccaaagggt ccgcctgttt tggtcttgga gtcaaatgtt cctgcgctgt
      721 ggaaagccag tcgcaggatc agtggggcgc atttcttttc tgcgatgaat ccc
//
