LOCUS       sequence-134846-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression of 
            3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target
            sequence (U6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE401E
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctatttcta gctctaaaac cgagaccggc ttattatgca cgcttaaaaa taataaaagc
       61 agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag
      121 ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc
      181 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
      241 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
      301 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
      361 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
      421 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
      481 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
      541 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
      601 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
      661 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
      721 ctcgctctct ccagg
//
