LOCUS       sequence-134849-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  humanSTARR-seq validation vector with Gateway cassette upstream of 
            the firefly luciferase gene and the SCP1 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pGL4-Gateway-SCP1
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(88..212)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      349..358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
       61 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
      121 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      181 gtttctcgtt cagctttctt gtacaaagtg gtagatctcc ctagggtact tatataaggg
      241 ggtgggggcg cgttcgtcct cagtcgcgat cgaacactcg agccgagcag acgtgcctac
      301 ggaccgggct agcgacctaa agcttggcaa tccggtactg ttggtaaagc caccatggaa
      361 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
      421 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
      481 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
      541 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
      601 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
      661 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
      721 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
      781 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
      841 agcatgtaca ccttcgtgac ttcccattt
//
