LOCUS       sequence-134850-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic Ca Biosensor, Excitation: 433 / 515, 
            Emission: 475 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     ECFP/Venus cytosolic calcium biosensor
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YC 3.6 Cameleon
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     88..109
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(212..233)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(377..454)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
       61 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      121 ccccggtgaa cagctcctcg cccttgctca ccatgccacc gctgccaccc ttgtacagct
      181 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      241 gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca
      301 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      361 cgccgtccat gagctccagt gccccggagc tggagatctt cttgaaccgg ttggcagcgc
      421 tgacggcaat gaagtttttc ttccagcgcc tcttcccccc ctttgctgtc atcatttgta
      481 caaactcttc atagtttact tggccatcac catcgatatc tgcttccctt atcatttcat
      541 caacttcttc atctgttaac ttctccccga ggtttgtcat gacgtgacgt aactgagcag
      601 cgctgatgta gccgttccca tccttgtcaa aaacacggaa tgcttctcgg atttcctctt
      661 cgctgtctgt gtccttcatt tttctagcca tcatagtaag aaattcagga aagtaaatcg
      721 ttccattgcc atcagcatcg acttcattga tcatatcctg caattctgct tccgttgggt
      781 tt
//
