LOCUS       sequence-134858-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  There is no gene/insert. It is a backbone plasmid that others can 
            use to insert sgRNAs of interest. The cloning site for this BsmBI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     pLenti-sgRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-sgRNA
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..252
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4..24
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     175..194
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
       61 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      121 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      181 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      241 ggacgaaaca ccggagacgg ttgtaaatga gcacacaaaa tacacatgct aaaatattat
      301 attctatgac ctttataaaa tcaaccaaaa tcttcttttt aataacttta gtatcaataa
      361 ttagaatttt tatgttcctt tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg
      421 ctagttttag taactcgcgt tgttttcttc acctttaata atagctactc caccacttgt
      481 tcctaagcgg tcagctcctg cttcaatcat tttttgagca tcttcaaatg ttctaactcc
      541 accagctgct ttaactaaag cattgtcttt aacaactgac ttcattagtt taacatcttc
      601 aaatgttgca cctgattttg aaaatcctgt tgatgtttta acaaattcta atccagcttc
      661 aacagctatt tcacaagctt tcatgatttc ttcttttgtt aataaacaat tttccataat
      721 acatttaaca acatgtgatc cagctgcttt ttttacagct ttcatgtctt ctaaaactaa
      781 ttcataattt ttgtctttta atgcaccaat atttaatacc atatcaattt ctgttgcacc
      841 atctttaatt gcttcagaaa cttcgaatgc ttttgtagct gttgtgcatg cac
//
