LOCUS       sequence-134859-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression fo Slc1a5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Nakaya M, Xiao Y, Zhou X, Chang JH, Chang M, Cheng X, Blonska M, Lin
            X, Sun SC
  TITLE     Inflammatory T cell responses rely on amino acid transporter ASCT2 
            facilitation of glutamine uptake and mTORC1 kinase activation.
  JOURNAL   Immunity. 2014 May 15;40(5):692-705. doi: 
            10.1016/j.immuni.2014.04.007. Epub 2014 May 1.
  PUBMED    24792914
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2014.04.007"; journalName: "Immunity"; date: 
            "2014-05-15- 15"; volume: "40"; issue: "5"; pages: "692-705"
COMMENT     Sequence Label: pCLXSN(GFP)-HA-Slc1a5 (mouse)
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(81..794,795..797,798..800))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(84..794,795..797,798..800))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(126..147)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     475..494
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     734..755
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aaatctttta ttttatcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
       61 agaaggcgat gcgatgcatc ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      121 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      181 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      241 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      301 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      361 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      421 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      481 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      541 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
      601 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      661 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
      721 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      781 tcctcgccct tgctcaccat ggtatcgatg ctcatcctgt ctcttgatca gatccgcctc
      841 gacctgcagc ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
      901 ctggaatagc tcaga
//
