LOCUS       sequence-134860-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  There is no gene/insert. It is a backbone plasmid that others can 
            use to insert sgRNAs of interest. The cloning site for this BsmBI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     pLenti-sgRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-sgRNA
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    263..380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(428..503)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc
       61 ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct
      121 tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga
      181 tgtgcgctct gcccactgac gggcaccgga gccactcgag ccggcgccaa agtggatctc
      241 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
      301 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
      361 tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtctcaag atctagaatt
      421 caaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      481 cttgctattt ctagctctaa aacagagacg tacaaaaaag agcaagaagc taaaaaagat
      541 ttaaaaatta tttttagcgc agttaatgga acaggaacta aatttacccc aaaaatatta
      601 cgtgaatcag gatataacgt tattgaggtt gaagagcatg catttgaaga tgaaacattt
      661 aaaaatgttg taaatccaaa tccagaattt gatcctgcat gaaaaatacc gcttgaatat
      721 ggtattaaac atgatgcaga tattattatt atgaatgacc cagatgctga cagatttgga
      781 atggcaataa aacatgatgg tcattttgta agattagatg gaaatcaaac aggaccaatt
      841 ttaattgatt gaaa
//
