LOCUS       sequence-134863-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK240
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..200)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQL"
     CDS             complement(231..824)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgaag ctgccacaag
       61 gcaggaacgt tggatggttc ccatcctgcc aaggaggtgt ggggctgcaa acatttatac
      121 gtcttgccgt tatatgtgac caattgtccc gcagtataag ctgtgttgac ctgccaagcg
      181 gatacaccag gatttgtcgt gaggcctgag ttcagaccgg tgaggccagt agcgtggctg
      241 acgaacccgt tcgtgataaa cgcgtggtct gccgtgtcga cacgaaggct atacaccggc
      301 tgcacgccgg cgtcggtgac actggcgact ttcgcgtagt agaaccgccc gtcggtcagc
      361 tcgtcggcga tagcttgggc gtccgggtct cggtggtgtg cttccaagaa acgcaccagt
      421 ccagggacgc cgactgtgta ggttgtgggc gcaaattcgg gtttcccgcg ggcaaaacct
      481 gcacagtcga cgctgaatgc gctgcgttga atcaccgcgt aatcgcccgg cttgatttcg
      541 tcgatcagct tccacagcag ggtcggcacc ccggcgacgt cgaccaaaca caacaacggg
      601 tggttcgcgg tgcccgtcac acgcagacct tcgaccgtac gcaccgtgta caccggatgc
      661 tcgccggagt ggaacagccg gtcggcgagc acgggattgc catgccggtc aaggactttc
      721 aggtcgatgg cgttgtcact gttgggccgc gcacccggca cgatgtcggc gatgcgtacc
      781 gactcgccct cgggtagggc aactagtgca tctcccgtga tgcagcgttt ggcaagcggc
      841 acatagtcac gctgcggtgc gccaatataa agctggcgag ggcggctgat acgctggccc
      901 ggttcttcaa tcatttcctt ccactggctc acccagccgg tggtgcgggc tacggcaaac
      961 agcacagtaa acatactggt ggggatgccc atggccttga
//
