LOCUS       sequence-134873-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli acetate kinase with an N-terminal His6 tag 
            (H6AckA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Murphy JR, Mullins EA, Kappock TJ
  TITLE     Functional Dissection of the Bipartite Active Site of the Class I 
            Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase.
  JOURNAL   Front Chem. 2016 May 23;4:23. doi: 10.3389/fchem.2016.00023. 
            eCollection 2016.
  PUBMED    27242998
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Chem."; date: "2016-05-23"; volume: "4:23. doi"; pages: " 
            10.3389/fchem.2016.00023. eCollection 2016"
COMMENT     Sequence Label: pJK667
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtc
       61 aggcagtcag gcggctcgcg tcttgcgcga taaccagttc ttcgttggtt gggataacca
      121 ccgcaggacg ggtaccttct ttgttgatga aaccagattt gccgaaacgt gcagccaggt
      181 tgcgttcatg atcaacttca aagcccagca cgcccagttt gcccagagac agttcacgaa
      241 ccatcgcggc attttcaccg ataccaccag tgaatacaac agcgtccaga cgaccatcca
      301 tcagcgcagt gtaggcaccg atgtatttcg ccaggcggtg gcagtaaacg tccattgcgc
      361 gcttcgcgtc ttctttcgtc gcgtagttgt cttcaacata gcggcagtcg ctggtcactt
      421 cggtcagacc cagcaggcca gactctttgg tcagcagttt gttgattgcg tcaacgctca
      481 tgcccagggt gtcgtgcagg tggaagatga tcgccggatc gatatcacca gaacgggtac
      541 ccatgaccag accttccagc ggggtcaggc ccatagaggt gtcaacgcat ttaccgttgc
      601 ggatagcaga aacggaacca ccgttgccca ggtggcaggt gatgatgttc agttcttcta
      661 ccggtttgtt cagcattttt gccgcttcct gggttacata gaagtggctg gtgccgtgcg
      721 cgccgtaacg acggatgccg tgctctttgt acaggttgta aggcagggcg tagaggtaag
      781 actcttccgg catagtctgg tggaacgcgg tgtcaaatac agcaacgttt ttgtctttca
      841 gctgtgggaa agatttcaga gcttcttcga taccgatcag gtgagccggg ttgtgcagcg
      901 gtgcaaaaga agctgcatct ttgataccct gaataacaga ctcatcgatc actacggagc
      961 tggtatactt ttcgccgccg tgtacgatac ggtgaccgat agcagtcagc tgcgcagaca
     1021 gttctggttt ttgtgccaga atagtattaa cgataaagtt gagcgcttcg ctgtgagcgg
     1081 cgcctgcacc taaagccgct tcctgtttat tgccgtccat tttccatttg atacgtgctt
     1141 ca
//
