LOCUS       sequence-134874-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK220
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttgtcgac ggagctcgaa ttcgtgattg agctcgactt cttatttgcg tagtctgacc
      121 tcttctaccg catgattagc acttttcgtc aggattaaac tggcgcgctc acgagtaggt
      181 agaatatttt gctttaagtt cagccagttg atctctttcc acaatgtcat ggcagtctta
      241 atcgcttctt ctttagttaa tttcgcgtag ttatgaaaat aggaatccgg gtcggtaaaa
      301 gccccttcgc ggaatttcag aaaacggttg atataccatg tctgaagtaa gtcttccggt
      361 gcatcaacat atatcgaaaa atcgacaaaa tcagaaacaa atacatgatg tggatcgtgt
      421 ggataatcca tcccgctctg taagacattt aacccttcaa gaattaaaat atcaggctga
      481 acaaccgttt tatctccatc cgggatcaca tcataaataa gatgtgagta aacaggtgct
      541 gtaacgtttg gcacgccgga tttgagatcg gaaacaaact tcaccaggtg atgcatatca
      601 tacgattccg ggaagccttt cttcttcatc agaccacgtt ctttcagaac ctgattaggg
      661 tgaaggaagc catctgtagt gatcagttca acacgacgat gttccggcca acggcttaat
      721 agcgcctgca atacacgggc ggttgtactt ttccccaccg cgacactgcc agcaatactg
      781 ataatgtaag gaatgcgttg cccgttggta ccaagaaact gttccagaac tgcctgacgg
      841 cgcagattcg agcttatata gaagttcagc aaacgtgaca aaggtaaata gatctcggca
      901 acttcttcta acgagagatc ttcattaata cctttgagac gggcgatctc atcttccgat
      961 aacgtcatag gtacggaatc acgcagagct gcccactggt tgcggtcaaa ctgtaggtaa
     1021 ggcgtcatta acgtttgatc ttttatactc atatggctgc c
//
