LOCUS       sequence-134875-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Thermoplasma acidophilum citrate synthase with C-terminal 
            His6 tag, His222>Gln mutant (TpCSH6-H222Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Murphy JR, Donini S, Kappock TJ
  TITLE     An active site-tail interaction in the structure of 
            hexahistidine-tagged Thermoplasma acidophilum citrate synthase.
  JOURNAL   Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1292-9. 
            doi: 10.1107/S2053230X15015939. Epub 2015 Sep 23.
  PUBMED    26457521
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr F Struct Biol Commun."; date: "2015-10-1"; volume: 
            "71(Pt 10):1292-9. doi"; pages: " 10.1107/S2053230X15015939. Epub 
            2015 Sep 23"
COMMENT     Sequence Label: pJK511
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacctttc tttcagcgat gggtacgtac tttctctctg caggaccgac gtaaacggct
      121 ctcggcctaa tcagtctctg ctgttcctcc acgtactcta tgaagtgtgc ctgccaccct
      181 gtaaccctgg atagcgcgaa gagcgccgtg tatatgttgt tcctcagcgg gaatcctatg
      241 gacatgtata ctattccgga gaagtaatcc gtgttcggat atatgccctt tgaaccgaat
      301 gccttgatgc caaagtcttc aagcttcgtg gcgatctcat ataccttgtg cacctctggc
      361 ttcttgctgg agagtttctc agcgatgccc ttgaatatct ttgcccttgg atcgtaggtc
      421 ttgtataccc tgtggccgaa gcccatgagt ctcttctttc cgtttattat gttgtcgttg
      481 aaccacttct ccaccatggc gggatccttt atctcatcga actgcgctat tgctgcctca
      541 gctgcaccgc cttgcagcgg acccttgagg gccgcaagag ctgcagttat gccggagtac
      601 atgtctgaga gggttgatac cgcgaccaag cctgccgtgg tagatgcagg cacctcatgg
      661 tctgtgtaga gaatcagtgc cgtattcatg gcgtctatct cttcctttgt tgccttcctt
      721 ccaaacgcag cgttcaggaa gctctcagcg tagctgtctg atggtttcgg aagctcggcc
      781 ggcatgttca ttatgtgcct gtacacgttc acggttatcg ctgacatcct gccgatcatt
      841 tctgctgcaa cgtctctgtc cgtatccttg ttccacttga actttgtctc cgatgctgcc
      901 atggctgcaa ccgcggccat ctgcattgcc acagcatccg attcccttgg cagctgcctt
      961 atggcgttta tgacaaaatc tggtatctta taacccttct ggacggtttc cttgtatttt
     1021 ctgagctcct gttcagttgg aaggttccca tacaggaaca gatactgga
//
