LOCUS       sequence-134876-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD287 (mKate2^SEC^3xMyc
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    287..308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(323..328,329..346,347..353)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    361..377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     366..388
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     385..401
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     385..401
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
       61 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      181 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      301 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      361 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgttatc gatttcctag
      421 gaacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
      481 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg ttcggtaagc
      541 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
      601 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc cgacgagaac
      661 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      721 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
      781 ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat
      841 cgg
//
