LOCUS       sequence-134881-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Mullins EA, Starks CM, Francois JA, Sael L, Kihara D, Kappock TJ
  TITLE     Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile 
            Acetobacter aceti has a distinctive electrostatic surface and 
            inherent acid stability.
  JOURNAL   Protein Sci. 2012 May;21(5):686-96. doi: 10.1002/pro.2054. Epub 2012
            Mar 29.
  PUBMED    22374910
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2054"; 
            journalName: "Protein Sci"; date: "2012-05"; volume: "21"; issue: 
            "5"; pages: "686-96"
COMMENT     Sequence Label: pJK597
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgtcaact ccacttcaag
      121 gaattaaagt tctcgatttc accggtgtgc aatctggccc atcttgtact caaatgctgg
      181 cctggtttgg cgctgacgtt attaaaattg aacgtcccgg cgttggtgac gtaacgcgtc
      241 accagctgcg agatattcct gatatcgatg cgctttactt caccatgctt aacagtaaca
      301 aacgttctat tgagttaaat accaaaacag cggaaggcaa agaggtaatg gaaaagctga
      361 tccgcgaagc tgatatctta gtcgagaact ttcatccagg ggccattgat cacatgggct
      421 tcacctggga gcatattcaa gaaatcaatc cacgtctgat ttttggttcg atcaaagggt
      481 ttgatgagtg ttcgccttat gtgaatgtaa aagcctatga aaacgttgct caggcagcgg
      541 gtggcgcggc atccactacg ggtttttggg atggtccgcc gctggtaagc gctgcagcgt
      601 tgggtgacag caacaccgga atgcatttgc tgatcggttt acttgctgct ttgctgcatc
      661 gcgaaaaaac ggggcgtggg caacgagtca ccatgtcaat gcaggatgcc gtattgaacc
      721 tttgccgcgt gaaattacgt gaccagcagc gtctcgataa attgggttat ctggaagaat
      781 acccgcagta tccgaatggt acatttggtg atgcagttcc ccgcggtggt aatgcaggtg
      841 gtggcggtca gcctggctgg atcctgaaat gtaaaggctg ggaaaccgat cctaacgcct
      901 atatttattt cactattcag gagcaaaact gggaaaacac ctgtaaagcc atcggcaaac
      961 cagaatggat taccgatccg gcatacagta cagcccatgc acgacagcca catattttcg
     1021 atatttttgc tgaaatcgaa aaatacactg tcactattga taaacatgaa gcggtggcct
     1081 atttgactca gtttgatatt ccttgtgcac cggttttaag
//
