LOCUS       sequence-134882-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses hM4D and a green fluorescent label from a Cre-dependent
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Atasoy D, Betley JN, Su HH, Sternson SM
  TITLE     Deconstruction of a neural circuit for hunger.
  JOURNAL   Nature. 2012 Aug 9;488(7410):172-7. doi: 10.1038/nature11270.
  PUBMED    22801496
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-08-9- 9"; volume: "488"; issue: "7410"; pages:
            "172-7"
COMMENT     Sequence Label: rAAV-CAG::FLEX-rev:: hM4D-2a-GFP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    164..197
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 caaattttgt aatccagagg ttgattatcg ataagctaat tcgaacctct tcgagggaca
       61 taacttcgta taatgtatgc tatacgaagt tatgggtcga tggtgagatc tggactagag
      121 ggtcgatggt gatgcttgga tcaaaatagg aagaatccca atgataactt cgtataaagt
      181 atcctatacg aagttatact agtaccatgg ccaacttcac acctgtcaat ggcagctcgg
      241 gcaatcagtc cgtgcgcctg gtcacgtcat catcccacaa tcgctatgag acggtggaaa
      301 tggtcttcat tgccacagtg acaggctccc tgagcctggt gactgtcgtg ggcaacatcc
      361 tggtgatgct gtccatcaag gtcaacaggc agctgcagac agtcaacaac tacttcctct
      421 tcagcctggc gtgtgctgat ctcatcatag gcgccttctc catgaacctc tacaccgtgt
      481 acatcatcaa gggctactgg cccctgggcg ccgtggtctg cgacctgtgg ctggccctgg
      541 actgcgtggt gagcaacgcc tccgtcatga accttctcat catcagcttt gaccgctact
      601 tctgcgtcac caagcctctc acctaccctg cccggcgcac caccaagatg gcaggcctca
      661 tgattgctgc tgcctgggta ctgtccttcg tgctctgggc gcctgccatc ttgttctggc
      721 agtttgtggt gggtaagcgg acggtgcccg acaaccagtg cttcatccag ttcctgtcca
      781 acccagcagt gacctttggc acagccattg ctggcttcta cctgcctgtg gtcatcatga
      841 cggtgctgta catccacatc tccctg
//
