LOCUS       sequence-134889-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression 
            3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, 
            TRY, and CPC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE2E-TRI
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(307..327)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(334..399)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atcgtcaacc ttcgccatct cgttggagaa aatctcctgc aggtagcaga tgcgattctt
       61 cctgcgcgtg taccgcctgc gggcggtgcg cttgagccgc gtagcctcag ccgtctcgcc
      121 gctgtcgaac aggagagcgc caatgagatt cttcttgatg gaatgccgat cggtgttgcc
      181 caggaccttg aacttctttg agggcacctt gtactcgtcg gtgatcacgg cccagccaac
      241 agagttagtc ccaatatcga ggccgatcga gtacttcttg tcagcagctg gcaccccgtg
      301 gatgccaacc ttcctcttct tcttcggagc catcttgtca tcatcatcct tgtaatcaat
      361 gtcgtggtcc ttgtaatccc cgtcgtggtc cttgtaatcc atctagattt ctcaacagat
      421 tgataaggtc gaaagaagaa agagtagaag ttgctttggt tgatttgttt gtgatgagaa
      481 ttgtgtgtta actttagaga gtttatatag agacagtaga gacttgactg ttacaaataa
      541 atggtagagt gaaagcccaa gtaattacga gggaagctca ttagattgtg agataattag
      601 tgcttagtgg tttagtgggt aaactaggtg taattactct attaaaagct gcgtaattag
      661 tgggtctgtt taggggctaa tgggccggat tagaatcact cagtctgaaa ctcgtttgat
      721 ttagttcaat agaaccaaat cagctttacc atcaaa
//
