LOCUS       sequence-134892-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression 
            3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, 
            TRY, and CPC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE2E-TRI
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        423..498
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atcatttctt cttagctttt tttcttcttc ttcgttcata cagttttttt ttgtttatca
       61 gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc attcggagtt
      121 tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg aaccttcaag
      181 aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc aaaaggcccc
      241 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt
      301 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa
      361 acgaagctga gtttatatac agctagagtc gaagtagtga ttgaatatct ctctatctcc
      421 tcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      481 gtggcaccga gtcggtgctt ttttttgcaa aattttccag atcgatttct tcttcctctg
      541 ttcttcggcg ttcaatttct ggggttttct cttcgttttc tgtaactgaa acctaaaatt
      601 tgacctaaaa aaatctcaaa taatatgatt cagtggtttt gtacttttca gttagttgag
      661 ttttgcagtt ccgatgagat aaaccaatat taatccaaac tactgcagcc tgacagacaa
      721 atgaggatgc aaacaatt
//
