LOCUS       sequence-134895-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli acetate kinase with an N-terminal His6 tag 
            (H6AckA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Murphy JR, Mullins EA, Kappock TJ
  TITLE     Functional Dissection of the Bipartite Active Site of the Class I 
            Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase.
  JOURNAL   Front Chem. 2016 May 23;4:23. doi: 10.3389/fchem.2016.00023. 
            eCollection 2016.
  PUBMED    27242998
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Chem."; date: "2016-05-23"; volume: "4:23. doi"; pages: " 
            10.3389/fchem.2016.00023. eCollection 2016"
COMMENT     Sequence Label: pJK667
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgtcgagtaa gttagtactg
      121 gttctgaact gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa
      181 gagtaccttt ctggtttagc cgaatgtttc cacctgcctg aagcacgtat caaatggaaa
      241 atggacggca ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc
      301 aactttatcg ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct
      361 atcggtcacc gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag
      421 tctgttattc agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac
      481 ctgatcggta tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct
      541 gtatttgaca ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgcct
      601 tacaacctgt acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc
      661 tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc
      721 acctgccacc tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac
      781 acctctatgg gcctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc
      841 gatccggcga tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac
      901 aaactgctga ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc
      961 tatgttgaag acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc
     1021 caccgcctgg cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct
     1081 gttgtattca ctggtggtat cggtgaaaat gccg
//
