LOCUS       sequence-134897-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK220
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgagtataaa agatcaaacg
      121 ttaatgacgc cttacctaca gtttgaccgc aaccagtggg cagctctgcg tgattccgta
      181 cctatgacgt tatcggaaga tgagatcgcc cgtctcaaag gtattaatga agatctctcg
      241 ttagaagaag ttgccgagat ctatttacct ttgtcacgtt tgctgaactt ctatataagc
      301 tcgaatctgc gccgtcaggc agttctggaa cagtttcttg gtaccaacgg gcaacgcatt
      361 ccttacatta tcagtattgc tggcagtgtc gcggtgggga aaagtacaac cgcccgtgta
      421 ttgcaggcgc tattaagccg ttggccggaa catcgtcgtg ttgaactgat cactacagat
      481 ggcttccttc accctaatca ggttctgaaa gaacgtggtc tgatgaagaa gaaaggcttc
      541 ccggaatcgt atgatatgca tcacctggtg aagtttgttt ccgatctcaa atccggcgtg
      601 ccaaacgtta cagcacctgt ttactcacat cttatttatg atgtgatccc ggatggagat
      661 aaaacggttg ttcagcctga tattttaatt cttgaagggt taaatgtctt acagagcggg
      721 atggattatc cacacgatcc acatcatgta tttgtttctg attttgtcga tttttcgata
      781 tatgttgatg caccggaaga cttacttcag acatggtata tcaaccgttt tctgaaattc
      841 cgcgaagggg cttttaccga cccggattcc tattttcata actacgcgaa attaactaaa
      901 gaagaagcga ttaagactgc catgacattg tggaaagaga tcaactggct gaacttaaag
      961 caaaatattc tacctactcg tgagcgcgcc agtttaatcc tgacgaaaag tgctaatcat
     1021 gcggtagaag aggtcagact acgcaaataa
//
