LOCUS       sequence-134901-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Periplasmic expression of APETx2 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Anangi R, Rash LD, Mobli M, King GF
  TITLE     Functional expression in Escherichia coli of the disulfide-rich sea 
            anemone peptide APETx2, a potent blocker of acid-sensing ion channel
            3.
  JOURNAL   Mar Drugs. 2012 Jul;10(7):1605-18. doi: 10.3390/md10071605. Epub 
            2012 Jul 23.
  PUBMED    22851929
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.3390/md10071605"; journalName: "Mar Drugs"; date: "2012-07"; 
            volume: "10"; issue: "7"; pages: "1605-18"
COMMENT     Sequence Label: pLicC-MBP-APETx2
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     285..304
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     392..411
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        392..410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    411..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             450..472
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             564..581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      181 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      241 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      301 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      361 aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga
      421 gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata
      481 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
      541 ccgcctcggc tctcgccaaa atccatcacc atcaccatca cgaagaaggt aaactggtaa
      601 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga
      661 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac
      721 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg
      781 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
      841 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg
      901 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct
      961 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gc
//
