LOCUS       sequence-134903-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Thermoplasma acidophilum citrate synthase with C-terminal 
            His6 tag, His222>Gln mutant (TpCSH6-H222Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Murphy JR, Donini S, Kappock TJ
  TITLE     An active site-tail interaction in the structure of 
            hexahistidine-tagged Thermoplasma acidophilum citrate synthase.
  JOURNAL   Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1292-9. 
            doi: 10.1107/S2053230X15015939. Epub 2015 Sep 23.
  PUBMED    26457521
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr F Struct Biol Commun."; date: "2015-10-1"; volume: 
            "71(Pt 10):1292-9. doi"; pages: " 10.1107/S2053230X15015939. Epub 
            2015 Sep 23"
COMMENT     Sequence Label: pJK511
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgccaga aactgaagaa
       61 attagcaaag ggcttgagga tgtcaatata aagtggacaa ggctcacgac catcgacgga
      121 aataagggaa tcctgcgata cgggggctat tctgtcgaag atataattgc ctctggggca
      181 caggatgagg agatccagta tctgttcctg tatgggaacc ttccaactga acaggagctc
      241 agaaaataca aggaaaccgt ccagaagggt tataagatac cagattttgt cataaacgcc
      301 ataaggcagc tgccaaggga atcggatgct gtggcaatgc agatggccgc ggttgcagcc
      361 atggcagcat cggagacaaa gttcaagtgg aacaaggata cggacagaga cgttgcagca
      421 gaaatgatcg gcaggatgtc agcgataacc gtgaacgtgt acaggcacat aatgaacatg
      481 ccggccgagc ttccgaaacc atcagacagc tacgctgaga gcttcctgaa cgctgcgttt
      541 ggaaggaagg caacaaagga agagatagac gccatgaata cggcactgat tctctacaca
      601 gaccatgagg tgcctgcatc taccacggca ggcttggtcg cggtatcaac cctctcagac
      661 atgtactccg gcataactgc agctcttgcg gccctcaagg gtccgctgca aggcggtgca
      721 gctgaggcag caatagcgca gttcgatgag ataaaggatc ccgccatggt ggagaagtgg
      781 ttcaacgaca acataataaa cggaaagaag agactcatgg gcttcggcca cagggtatac
      841 aagacctacg atccaagggc aaagatattc aagggcatcg ctgagaaact ctccagcaag
      901 aagccagagg tgcacaaggt atatgagatc gccacgaagc ttgaagactt tggcatcaag
      961 gcattcggtt caaagggcat atatccgaac acggattact tctccggaat agtatacatg
     1021 tccataggat tcccgctgag gaacaacata tacacggcgc tcttcgcgct atccagggtt
     1081 acagggtggc ag
//
