LOCUS       sequence-134904-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Periplasmic expression of APETx2 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Anangi R, Rash LD, Mobli M, King GF
  TITLE     Functional expression in Escherichia coli of the disulfide-rich sea 
            anemone peptide APETx2, a potent blocker of acid-sensing ion channel
            3.
  JOURNAL   Mar Drugs. 2012 Jul;10(7):1605-18. doi: 10.3390/md10071605. Epub 
            2012 Jul 23.
  PUBMED    22851929
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.3390/md10071605"; journalName: "Mar Drugs"; date: "2012-07"; 
            volume: "10"; issue: "7"; pages: "1605-18"
COMMENT     Sequence Label: pLicC-MBP-APETx2
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(211..231)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(256..279)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cttattaatc tgccggtgta caacaggtgc ccagaaaata
      121 acgacagcta ccggtataac cacgatcggt cggacagctc ggacgataaa accaataaat
      181 gcctttgcta ttaccacagc tacatgcggt gccctgaaaa tacagatttt cggtacctgg
      241 gatatcgtaa tcagtctgcg cgtctttcag ggcttcatcg acagtctgac gaccgctggc
      301 ggcgttgatc accgcagtac gcacggcata ccagaaagcg gacatctgcg ggatgttcgg
      361 catgatttca cctttctggg cgttttccat ggtggcggca atacgtggat ctttcgccaa
      421 ctcttcctcg taagacttca gcgctacggc acccagcggt ttgtctttat taaccgcttc
      481 cagaccttca tcagtcagca gatagttttc gaggaactct tttgccagct ctttgttcgg
      541 actggcggcg ttaatacctg cgctcagcac gccaacgaac ggtttggatg gttgaccctt
      601 gaaggtcggc agtaccgtta caccataatt cactttgctg gtgtcgatgt tggaccatgc
      661 ccacgggccg ttgatggtca tcgctgtttc gcctttatta aaggcagctt ctgcgatgga
      721 gtaatcggtg tctgcattca tgtgtttgtt tttaatcagg tcaaccagga aggtcagacc
      781 cgctttcgcg ccagcgttat ccacgcccac gtctttaatg tcgtacttgc cgttttcata
      841 cttgaacgca taacccccgt cagcagcaat cagcggccag gtgaagtacg gttcttgcag
      901 gttgaacatc agcgcgctct tacctttcgc tttcagttct ttatccagcg ccgggatctc
      961 ttcccaggtt t
//
