LOCUS       sequence-134908-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human TDG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar 
            P
  TITLE     3CAPS - a structural AP-site analogue as a tool to investigate DNA 
            base excision repair.
  JOURNAL   Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.
  PUBMED    26733580
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jan 4. pii: gkv1520."
COMMENT     Sequence Label: pET28-hTDG
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             171..188
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
      181 gcggcagcca tatggctagc gaagcggaga acgcgggcag ctattccctt cagcaagctc
      241 aagcttttta tacgtttcca tttcaacaac tgatggctga agctcctaat atggcagttg
      301 tgaatgaaca gcaaatgcca gaagaagttc cagccccagc tcctgctcag gaaccagtgc
      361 aagaggctcc aaaaggaaga aaaagaaaac ccagaacaac agaaccaaaa caaccagtgg
      421 aacccaaaaa acctgttgag tcaaaaaaat ctggcaagtc tgcaaaatca aaagaaaaac
      481 aagaaaaaat tacagacaca tttaaagtaa aaagaaaagt agaccgtttt aatggtgttt
      541 cagaagctga acttctgacc aagactctcc ccgatatttt gaccttcaat ctggacattg
      601 tcattattgg cataaacccg ggactaatgg ctgcttacaa agggcatcat taccctggac
      661 ctggaaacca tttttggaag tgtttgttta tgtcagggct cagtgaggtc cagctgaacc
      721 atatggatga tcacactcta ccagggaagt atggtattgg atttaccaac atggtggaa
//
