LOCUS       sequence-134909-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Pfu DNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Goldman S, Kim R, Hung LW, Jancarik J, Kim SH
  TITLE     Purification, crystallization and preliminary X-ray crystallographic
            analysis of Pyrococcus furiosus DNA polymerase.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8.
  PUBMED    9757114
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8."
COMMENT     Sequence Label: pET16B.Pfu
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..174
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             187..198
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gggccatcat catcatcatc atcatcatca tcacagcagc
      181 ggccatatcg aaggtcgtca tatgatttta gatgtggatt acataactga agaaggaaaa
      241 cctgttatta ggctattcaa aaaagagaac ggaaaattta agatagagca tgatagaact
      301 tttagaccat acatttacgc tcttctcagg gatgattcaa agattgaaga agttaagaaa
      361 ataacggggg aaaggcatgg aaagattgtg agaattgttg atgtagagaa ggttgagaaa
      421 aagtttctcg gcaagcctat taccgtgtgg aaactttatt tggaacatcc ccaagatgtt
      481 cccactatta gagaaaaagt tagagaacat ccagcagttg tggacatctt cgaatacgat
      541 attccatttg caaagagata cctcatcgac aaaggcctaa taccaatgga gggggaagaa
      601 gagctaaaga ttcttgcctt cgatatagaa accctctatc acgaaggaga agagtttgga
      661 aaaggcccaa ttataatgat tagttatgca gatgaaaatg aagcaaaggt gattacttgg
      721 aaaaacatag atcttccata cgttgaggtt gtatcaagcg agagagagat gataaagaga
      781 tttctcagga ttatcaggga gaaggatcct gacattatag ttacttataa tggagactca
      841 ttcgacttcc cacatttagc gaaaagggca gaaaa
//
