LOCUS       sequence-134911-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-RhoA-Q63L
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..84
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(121..306,307..309,310..702)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     regulatory      722..731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    735..869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(772..791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     826..845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattggccac catggagcag
       61 aagctgatct ccgaggagga cctgggatcc atggagtatg atgttcctga ttatgctcat
      121 atggctgcca tccggaagaa actggtgatt gttggtgatg gagcctgtgg aaagacatgc
      181 ttgctcatag tcttcagcaa ggaccagttc ccagaggtgt atgtgcccac agtgtttgag
      241 aactatgtgg cagatatcga ggtggatgga aagcaggtag agttggcttt gtgggacaca
      301 gctgggctgg aagattatga tcgcctgagg cccctctcct acccagatac cgatgttata
      361 ctgatgtgtt tttccatcga cagccctgat agtttagaaa acatcccaga aaagtggacc
      421 ccagaagtca agcatttctg tcccaacgtg cccatcatcc tggttgggaa taagaaggat
      481 cttcggaatg atgagcacac aaggcgggag ctagccaaga tgaagcagga gccggtgaaa
      541 cctgaagaag gcagagatat ggcaaacagg attggcgctt ttgggtacat ggagtgttca
      601 gcaaagacca aagatggagt gagagaggtt tttgaaatgg ctacgagagc tgctctgcaa
      661 gctagacgtg ggaagaaaaa atctggttgc cttgtcttgt gagaattcct gcagaagctt
      721 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      841 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcag
//
