LOCUS       sequence-134912-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human XRCC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar 
            P
  TITLE     3CAPS - a structural AP-site analogue as a tool to investigate DNA 
            base excision repair.
  JOURNAL   Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.
  PUBMED    26733580
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jan 4. pii: gkv1520."
COMMENT     Sequence Label: pET28-hXRCC1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atataccatg ggatccatgc cggagatccg cctccgccat gtcgtgtcct
      181 gcagcagcca ggactcgact cactgtgcag aaaatcttct caaggcagac acttaccgaa
      241 aatggcgggc agccaaggca ggcgagaaga ccatctctgt ggtcctacag ttggagaagg
      301 aggagcagat acacagtgtg gacattggga atgatggctc agctttcgtg gaggtgctgg
      361 tgggcagttc agctggaggc gctggggagc aagactatga ggtccttctg gtcacctcat
      421 ctttcatgtc cccttccgag agccgcagtg gctcaaaccc caaccgcgtt cgcatgtttg
      481 ggcctgacaa gctggtccgg gcagccgccg agaagcgctg ggaccgggtc aaaattgttt
      541 gcagccagcc ctacagcaag gactccccct ttggcttgag ttttgtacgg tttcatagcc
      601 ccccagacaa agatgaggca gaggccccgt cccagaaggt gacagtgacc aagcttggcc
      661 agttccgtgt gaaggaggag gatgagagcg ccaactctct gaggccgggg gctctcttct
      721 tcagccggat caacaagaca tccccagtca cagccagcga cccggcagga cctagctatg
      781 cagctgctac cctccaggct tctagtgctg cctcctcagc ctctccagtc tccagggcca
      841 taggcagcac ctccaagcc
//
