LOCUS       sequence-134914-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High efficiency Streptomyces genome editing by CRISPR/Cas9 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Huang H, Zheng G, Jiang W, Hu H, Lu Y
  TITLE     One-step high-efficiency CRISPR/Cas9-mediated genome editing in 
            Streptomyces.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 
            10.1093/abbs/gmv007. Epub 2015 Mar 3.
  PUBMED    25739462
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/abbs/gmv007"; journalName: "Acta Biochim Biophys Sin 
            (Shanghai)"; date: "2015-04"; volume: "47"; issue: "4"; pages: 
            "231-43"
COMMENT     Sequence Label: pKCcas9dO
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgccgccca gctgggagag gtcgatgcgg gtctcgtaca ggcccgtgat gctctggtgg
       61 atcagggtgg cgtcgagcac ctccttcgtg ctcgtgtacc gcttccggtc gatcgtggtg
      121 tcgaagtact tgaaggccgc gggggcgccg aggttggtca gcgtgaacag gtggatgatg
      181 ttctcggcct gctcccggat cggcttgtcg cggtgcttgt tgtacgcgga caggaccttg
      241 tccaggttcg cgtcggccag gatgacgcgc ttggagaact cggagatctg ctcgatgatc
      301 tcgtcgaggt agtgcttgtg ctgctccacg aacagctgct tctgctcgtt gtcctccggc
      361 gagcccttca gcttctcgta gtgcgaggcg aggtacagga agttgacgta cttgctcggc
      421 agcgccagct cgttgccctt ctgcagctcg cccgcgctgg cgagcatgcg cttccggccg
      481 ttctccagct cgaacaggga gtacttgggg agcttgatga tgaggtcctt cttcacctcc
      541 ttgtagccct tggcctccag gaagtcgatg gggttcttct cgaacgacga ccgctccatg
      601 atggtgatgc ccagcagctc cttcacggac ttcagcttct tgctcttgcc cttctccacc
      661 ttggcgacca cgaggaccga gtaggcgacg gtgggggagt cgaagccgcc gtacttcttg
      721 gggtcccagt ccttcttccg ggcgatcagc ttgtcgctgt tccgcttggg caggatggac
      781 tccttgctga agccgccggt ctggacctcc gtcttcttga cgatgttgac ctggggcatg
      841 gacagcacct tccggacggt cgcgaagtcg cggcccttgt cccacacgat ctcgcccgtc
      901 tcgccgttgg tctcgatg
//
