LOCUS       sequence-134943-        8592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Shakespear MR, Hohenhaus DM, Kelly GM, Kamal NA, Gupta P, Labzin LI,
            Schroder K, Garceau V, Barbero S, Iyer A, Hume DA, Reid RC, Irvine 
            KM, Fairlie DP, Sweet MJ
  TITLE     Histone deacetylase 7 promotes Toll-like receptor 4-dependent 
            proinflammatory gene expression in macrophages.
  JOURNAL   J Biol Chem. 2013 Aug 30;288(35):25362-74. doi: 
            10.1074/jbc.M113.496281. Epub 2013 Jul 12.
  PUBMED    23853092
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.496281"; journalName: "J Biol Chem"; date: 
            "2013-08-30- 30"; volume: "288"; issue: "35"; pages: "25362-74"
COMMENT     Sequence Label: mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)
FEATURES             Location/Qualifiers
     source          1..8592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2818..2859
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2869..2886
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2909..2926)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2915..3139
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3185..3613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3272..3291)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3482..3503
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3622..3642)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3627..3957
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3808..3943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3870..3889
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4005..4052
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4071..4469
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4627..4748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4664..4683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4718..4737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4797..4813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4797..4813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4810..4832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4821..4837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4845..4851,4852..4869,4870..4875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4890..4911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5028..5045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5199..5787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5279..5298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5958..6749,6750..6818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6581..6600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6819..6923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6998..7017)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7307..8485
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7538..8476
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8433..8453
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8502..8521
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8502..8520
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accatggacc tgcgggtggg ccagcggccc acggtggagc ccccaccaga gcctgcgctg
       61 ctgaccctgc aacaccccca acgcctgcac cgccatctct tcctggcagg cttacaccag
      121 caacagcgct cagccgagcc catgaggctc tccatggacc caccaatgcc ggagctgcag
      181 gggggacagc aggagcaaga acttcggcaa cttctcaata aagacaagag caagcgaagt
      241 gccgtagcca gcagtgtggt caagcagaag ctggctgaag tgatcctgaa gaaacagcag
      301 gcagcccttg agagaacagt ccatcccagc agccccagta ttccctacag aactcttgag
      361 cccttggaca cagagggtgc tgcccgctcc gtgcttagca gcttcctgcc tcctgttccc
      421 agcctgccca ctgaaccccc ggaacacttt cccttgcgta aaacagtgtc tgaacccaac
      481 ctgaagttgc gctacaaacc caagaaatcc ctggagagac gcaagaatcc cctgctcagg
      541 aaggagagtg ccccgcccag ccttcggagg aggcctgccg agacccttgg agattcctcc
      601 cccagtagta gcagcacacc cgcgtcaggg tgcagctccc ctaatgacag cgagcatggc
      661 cctaaccctg ccctaggctc agaggctgat ggtgaccgca ggacccattc aactttaggc
      721 cctcggggtc ctgtactggg gaacccccat gctcccctct tcctgcacca cggtctggag
      781 ccagaggctg ggggcacctt accctctcgc ctgcaaccca ttctcctgct ggacccctca
      841 gtctctcatg ccccactgtg gactgtgcct ggccttgggc ccttgccctt ccactttgcc
      901 cagcccttac tgaccaccga gcggctctct gggtcaggcc tccatcgacc acttaaccgg
      961 acccgctcag agcccctgcc ccccagcgcc acagcctccc ctctgctggc ccccctgcag
     1021 ccccgccagg atcggctcaa acctcacgtc cagctgatca agccagccat ctcccctccc
     1081 cagaggcctg ccaagcccag tgagaagccc cgactgcgac agataccctc ggctgaggac
     1141 ctagagacag atggtggggg agtgggacct atggcgaatg atggcctgga acatagggag
     1201 tcaggccgtg ggcctcctga gggcagaggc tccatttctc tgcagcagca ccaacaggtg
     1261 ccaccctggg agcagcagca tctagccggg cggctctctc agggaagccc cggggactcc
     1321 gtgctgatac ctctggccca ggttggacac cggcccctgt ccagaaccca gtcttcccca
     1381 gcagcacctg tctccatgct gagcccagag cccacctgtc agacccaagt cctcaacagc
     1441 tcagagacac ctgctacagg gctggtctat gactcggtga tgctgaaaca ccaatgttcc
     1501 tgtggagaca acagcaagca tcccgagcat gcaggccgca tccagagcat ctggtcccgg
     1561 ctgcaggaac ggggtctccg cagccagtgt gagtgtctcc gaggccgaaa ggcttcccta
     1621 gaggagctgc agtcagtcca ctctgaacgg cacgtgctcc tctacggcac gaacccactc
     1681 agccgcctca aactggataa cgggaagctt acaggactcc tggcacagcg gacgtttgtg
     1741 atgctaccct gtggcggggt tggggtcgat actgacacca tctggaacga gctgcattcc
     1801 tccaatgcag cccgctgggc tgcgggcagc gtcaccgacc ttgccttcaa agtagcttcc
     1861 cgagagctga agaacggctt tgctgtggtg cgacccccgg gacaccatgc agatcattct
     1921 acagccatgg gcttctgctt cttcaactcc gtggccatcg cctgccgaca gctacagcaa
     1981 cacggcaaag ccagcaagat cctcattgtt gactgggatg ttcaccatgg caacggcaca
     2041 cagcagactt tctaccagga ccccagtgtg ctctacattt cccttcatcg tcatgacgac
     2101 ggcaacttct tcccaggcag tggggccgtg gatgaggtgg gaactggcag tggcgagggc
     2161 ttcaatgtca acgtggcttg ggctgggggc ttggatccac ccatggggga tcctgagtac
     2221 ctggctgctt tcaggatagt ggtgatgccc attgcccgag agtttgctcc agacctggtc
     2281 ctggtgtctg ctgggtttga tgctgcggag ggtcacccag ccccactggg tggctaccat
     2341 gtttctgcca aatgttttgg gtacatgacg cagcagttga tgaacttggc aggaggcgcc
     2401 gtggtgttgg ccttagaggg tggacatgac ctcacggcca tctgtgatgc ctcggaggcc
     2461 tgtgtagctg ctcttctggg caacaaggtg gacccccttt cagaagaaag ctggaaacag
     2521 aaacccaacc tcagtgccat ccgctcgctg gaagctgtgg tcagggtgca caggaaatac
     2581 tggggctgca tgcagcgctt ggcctcctgt ccagactcct ggctacccag agtgccggga
     2641 gctgatgcag aagtggaagc cgtgaccgcg ctggcatccc tttctgtggg catcctggct
     2701 gaagacaggc cctcggagcg gctggtggaa gaggaagaac ccatgaacct caagggcaat
     2761 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     2821 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     2881 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     2941 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3001 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3061 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3121 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3181 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3241 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3301 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     3361 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3421 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3481 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3541 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3601 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3661 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3781 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3841 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     3901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3961 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     4021 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagc
     4081 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     4141 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     4201 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     4261 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     4321 ggggcatctt gagcccctgc ggacggtgtc gacaggtgct tctcgatctg catcctggga
     4381 tcaaagcgat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     4441 tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca ggactgacac
     4501 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4561 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4621 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4741 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     4801 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4861 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4921 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4981 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5041 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     5401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     5641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5941 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6001 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6061 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6121 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6181 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6241 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6301 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6361 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6421 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6481 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6541 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6601 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6661 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6721 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6781 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6841 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6901 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     6961 tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat
     7021 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     7081 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     7141 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     7201 tgactaggct tttgcaaaaa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     7261 tttgcagcta atggaccttc taggtcttga aaggagtgcc tcgtgaggct ccggtgcccg
     7321 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     7381 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     7441 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     7501 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     7561 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     7621 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     7681 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     7741 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     7801 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     7861 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     7921 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     7981 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     8041 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     8101 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     8161 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     8221 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     8281 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     8341 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     8401 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     8461 ttttcttcca tttcaggtgt cgtgaggaat tagcttggta ctaatacgac tcactatagg
     8521 gagacccaag ctggctaggt aagcttggta ccgagctcgg atccactagt ccagtgtggt
     8581 ggaattgccc tt
//
