LOCUS       sequence-134945-        8535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Shakespear MR, Hohenhaus DM, Kelly GM, Kamal NA, Gupta P, Labzin LI,
            Schroder K, Garceau V, Barbero S, Iyer A, Hume DA, Reid RC, Irvine 
            KM, Fairlie DP, Sweet MJ
  TITLE     Histone deacetylase 7 promotes Toll-like receptor 4-dependent 
            proinflammatory gene expression in macrophages.
  JOURNAL   J Biol Chem. 2013 Aug 30;288(35):25362-74. doi: 
            10.1074/jbc.M113.496281. Epub 2013 Jul 12.
  PUBMED    23853092
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.496281"; journalName: "J Biol Chem"; date: 
            "2013-08-30- 30"; volume: "288"; issue: "35"; pages: "25362-74"
COMMENT     Sequence Label: mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)
FEATURES             Location/Qualifiers
     source          1..8535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2806..2829
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2852..2869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2858..3082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3128..3556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3215..3234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3425..3446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3565..3585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3570..3900
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3751..3886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3813..3832
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3948..3995
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4014..4412
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4570..4691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4607..4626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4661..4680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4740..4756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4740..4756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4753..4775)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4764..4780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4788..4794,4795..4812,4813..4818))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4833..4854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4971..4988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5142..5730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5222..5241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5901..6692,6693..6761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6524..6543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6762..6866)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6941..6960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7250..8428
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7481..8419
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8376..8396
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8445..8464
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8445..8463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accatggacc tgcgggtggg ccagcggccc acggtggagc ccccaccaga gcctgcgctg
       61 ctgaccctgc aacaccccca acgcctgcac cgccatctct tcctggcagg cttacaccag
      121 caacagcgct cagccgagcc catgaggctc tccatggacc caccaatgcc ggagctgcag
      181 gggggacagc aggagcaaga acttcggcaa cttctcaata aagacaagag caagcgaagt
      241 gccgtagcca gcagtgtggt caagcagaag ctggctgaag tgatcctgaa gaaacagcag
      301 gcagcccttg agagaacagt ccatcccagc agccccagta ttccctacag aactcttgag
      361 cccttggaca cagagggtgc tgcccgctcc gtgcttagca gcttcctgcc tcctgttccc
      421 agcctgccca ctgaaccccc ggaacacttt cccttgcgta aaacagtgtc tgaacccaac
      481 ctgaagttgc gctacaaacc caagaaatcc ctggagagac gcaagaatcc cctgctcagg
      541 aaggagagtg ccccgcccag ccttcggagg aggcctgccg agacccttgg agattcctcc
      601 cccagtagta gcagcacacc cgcgtcaggg tgcagctccc ctaatgacag cgagcatggc
      661 cctaaccctg ccctaggctc agaggctgat ggtgaccgca ggacccattc aactttaggc
      721 cctcggggtc ctgtactggg gaacccccat gctcccctct tcctgcacca cggtctggag
      781 ccagaggctg ggggcacctt accctctcgc ctgcaaccca ttctcctgct ggacccctca
      841 gtctctcatg ccccactgtg gactgtgcct ggccttgggc ccttgccctt ccactttgcc
      901 cagcccttac tgaccaccga gcggctctct gggtcaggcc tccatcgacc acttaaccgg
      961 acccgctcag agcccctgcc ccccagcgcc acagcctccc ctctgctggc ccccctgcag
     1021 ccccgccagg atcggctcaa acctcacgtc cagctgatca agccagccat ctcccctccc
     1081 cagaggcctg ccaagcccag tgagaagccc cgactgcgac agataccctc ggctgaggac
     1141 ctagagacag atggtggggg agtgggacct atggcgaatg atggcctgga acatagggag
     1201 tcaggccgtg ggcctcctga gggcagaggc tccatttctc tgcagcagca ccaacaggtg
     1261 ccaccctggg agcagcagca tctagccggg cggctctctc agggaagccc cggggactcc
     1321 gtgctgatac ctctggccca ggttggacac cggcccctgt ccagaaccca gtcttcccca
     1381 gcagcacctg tctccatgct gagcccagag cccacctgtc agacccaagt cctcaacagc
     1441 tcagagacac ctgctacagg gctggtctat gactcggtga tgctgaaaca ccaatgttcc
     1501 tgtggagaca acagcaagca tcccgagcat gcaggccgca tccagagcat ctggtcccgg
     1561 ctgcaggaac ggggtctccg cagccagtgt gagtgtctcc gaggccgaaa ggcttcccta
     1621 gaggagctgc agtcagtcca ctctgaacgg cacgtgctcc tctacggcac gaacccactc
     1681 agccgcctca aactggataa cgggaagctt acaggactcc tggcacagcg gacgtttgtg
     1741 atgctaccct gtggcggggt tggggtcgat actgacacca tctggaacga gctgcattcc
     1801 tccaatgcag cccgctgggc tgcgggcagc gtcaccgacc ttgccttcaa agtagcttcc
     1861 cgagagctga agaacggctt tgctgtggtg cgacccccgg gacaccatgc agatcattct
     1921 acagccatgg gcttctgctt cttcaactcc gtggccatcg cctgccgaca gctacagcaa
     1981 cacggcaaag ccagcaagat cctcattgtt gactgggatg ttcaccatgg caacggcaca
     2041 cagcagactt tctaccagga ccccagtgtg ctctacattt cccttcatcg tcatgacgac
     2101 ggcaacttct tcccaggcag tggggccgtg gatgaggtgg gaactggcag tggcgagggc
     2161 ttcaatgtca acgtggcttg ggctgggggc ttggatccac ccatggggga tcctgagtac
     2221 ctggctgctt tcaggatagt ggtgatgccc attgcccgag agtttgctcc agacctggtc
     2281 ctggtgtctg ctgggtttga tgctgcggag ggtcacccag ccccactggg tggctaccat
     2341 gtttctgcca aatgttttgg gtacatgacg cagcagttga tgaacttggc aggaggcgcc
     2401 gtggtgttgg ccttagaggg tggacatgac ctcacggcca tctgtgatgc ctcggaggcc
     2461 tgtgtagctg ctcttctggg caacaaggtg gacccccttt cagaagaaag ctggaaacag
     2521 aaacccaacc tcagtgccat ccgctcgctg gaagctgtgg tcagggtgca caggaaatac
     2581 tggggctgca tgcagcgctt ggcctcctgt ccagactcct ggctacccag agtgccggga
     2641 gctgatgcag aagtggaagc cgtgaccgcg ctggcatccc tttctgtggg catcctggct
     2701 gaagacaggc cctcggagcg gctggtggaa gaggaagaac ccatgaacct caagggcaat
     2761 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcgacta caaagacgat
     2821 gacgacaagt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     2881 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2941 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3001 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3061 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3121 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3181 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3241 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc
     3301 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3361 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3421 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3481 gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3541 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3601 gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3661 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3721 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3781 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     3841 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3901 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     3961 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     4021 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     4081 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     4141 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     4201 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     4261 acaggggcat cttgagcccc tgcggacggt gtcgacaggt gcttctcgat ctgcatcctg
     4321 ggatcaaagc gatagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     4381 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga
     4441 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4501 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4561 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4681 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     4741 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4801 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4861 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4921 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4981 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5041 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5101 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5161 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5221 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5281 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     5341 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5401 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5461 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5521 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5581 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5641 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5701 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5761 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5821 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5881 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5941 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6001 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6061 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6121 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6181 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6241 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6301 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6361 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6421 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6481 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6541 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6601 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6661 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6721 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     6901 ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg
     6961 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     7021 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     7081 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     7141 tattgactag gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga
     7201 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gcctcgtgag gctccggtgc
     7261 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     7321 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     7381 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     7441 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     7501 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     7561 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     7621 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     7681 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     7741 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     7801 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     7861 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     7921 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     7981 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     8041 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     8101 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     8161 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     8221 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     8281 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     8341 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     8401 tttttttctt ccatttcagg tgtcgtgagg aattagcttg gtactaatac gactcactat
     8461 agggagaccc aagctggcta ggtaagcttg gtaccgagct cggatccact agtccagtgt
     8521 ggtggaattg ccctt
//
