LOCUS       sequence-134950-        7290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Shakespear MR, Hohenhaus DM, Kelly GM, Kamal NA, Gupta P, Labzin LI,
            Schroder K, Garceau V, Barbero S, Iyer A, Hume DA, Reid RC, Irvine 
            KM, Fairlie DP, Sweet MJ
  TITLE     Histone deacetylase 7 promotes Toll-like receptor 4-dependent 
            proinflammatory gene expression in macrophages.
  JOURNAL   J Biol Chem. 2013 Aug 30;288(35):25362-74. doi: 
            10.1074/jbc.M113.496281. Epub 2013 Jul 12.
  PUBMED    23853092
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.496281"; journalName: "J Biol Chem"; date: 
            "2013-08-30- 30"; volume: "288"; issue: "35"; pages: "25362-74"
COMMENT     Sequence Label: mHDAC7-Nterm (23-504) pEF6-V5
FEATURES             Location/Qualifiers
     source          1..7290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1516..1557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1567..1584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1607..1624)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1613..1837
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1883..2311
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1970..1989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2180..2201
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2320..2340)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2325..2655
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2506..2641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2568..2587
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2703..2750
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2769..3167
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3325..3446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3362..3381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3416..3435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3495..3511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3495..3511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3508..3530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3519..3535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3543..3549,3550..3567,3568..3573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3588..3609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3726..3743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3897..4485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3977..3996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4656..5447,5448..5516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5279..5298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5517..5621)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5696..5715)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6005..7183
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6236..7174
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7131..7151
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7200..7219
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7200..7218
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accatggacc tgcgggtggg ccagcggccc acggtggagc ccccaccaga gcctgcgctg
       61 ctgaccctgc aacaccccca acgcctgcac cgccatctct tcctggcagg cttacaccag
      121 caacagcgct cagccgagcc catgaggctc tccatggacc caccaatgcc ggagctgcag
      181 gggggacagc aggagcaaga acttcggcaa cttctcaata aagacaagag caagcgaagt
      241 gccgtagcca gcagtgtggt caagcagaag ctggctgaag tgatcctgaa gaaacagcag
      301 gcagcccttg agagaacagt ccatcccagc agccccagta ttccctacag aactcttgag
      361 cccttggaca cagagggtgc tgcccgctcc gtgcttagca gcttcctgcc tcctgttccc
      421 agcctgccca ctgaaccccc ggaacacttt cccttgcgta aaacagtgtc tgaacccaac
      481 ctgaagttgc gctacaaacc caagaaatcc ctggagagac gcaagaatcc cctgctcagg
      541 aaggagagtg ccccgcccag ccttcggagg aggcctgccg agacccttgg agattcctcc
      601 cccagtagta gcagcacacc cgcgtcaggg tgcagctccc ctaatgacag cgagcatggc
      661 cctaaccctg ccctaggctc agaggctgat ggtgaccgca ggacccattc aactttaggc
      721 cctcggggtc ctgtactggg gaacccccat gctcccctct tcctgcacca cggtctggag
      781 ccagaggctg ggggcacctt accctctcgc ctgcaaccca ttctcctgct ggacccctca
      841 gtctctcatg ccccactgtg gactgtgcct ggccttgggc ccttgccctt ccactttgcc
      901 cagcccttac tgaccaccga gcggctctct gggtcaggcc tccatcgacc acttaaccgg
      961 acccgctcag agcccctgcc ccccagcgcc acagcctccc ctctgctggc ccccctgcag
     1021 ccccgccagg atcggctcaa acctcacgtc cagctgatca agccagccat ctcccctccc
     1081 cagaggcctg ccaagcccag tgagaagccc cgactgcgac agataccctc ggctgaggac
     1141 ctagagacag atggtggggg agtgggacct atggcgaatg atggcctgga acatagggag
     1201 tcaggccgtg ggcctcctga gggcagaggc tccatttctc tgcagcagca ccaacaggtg
     1261 ccaccctggg agcagcagca tctagccggg cggctctctc agggaagccc cggggactcc
     1321 gtgctgatac ctctggccca ggttggacac cggcccctgt ccagaaccca gtcttcccca
     1381 gcagcacctg tctccatgct gagcccagag cccacctgtc agacccaagt cctcaacagc
     1441 tcagagacaa agggcaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag
     1501 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     1561 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     1621 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1681 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     1741 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     1801 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     1861 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1921 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1981 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     2041 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2101 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2161 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2221 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     2281 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     2341 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     2401 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2461 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2581 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2641 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     2701 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     2761 actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     2821 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     2881 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     2941 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     3001 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgtcga caggtgcttc
     3061 tcgatctgca tcctgggatc aaagcgatag tgaaggacag tgatggacag ccgacggcag
     3121 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc
     3181 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3241 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3301 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     3361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3421 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     3481 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4081 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4141 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4201 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4261 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4321 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4381 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4441 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4501 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5101 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5281 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5521 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5641 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa
     5701 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     5761 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     5821 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     5881 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
     5941 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgcctc
     6001 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     6061 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     6121 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     6181 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     6241 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     6301 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     6361 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     6421 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     6481 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     6541 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     6601 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     6661 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     6721 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     6781 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     6841 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     6901 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     6961 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     7021 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     7081 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     7141 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact
     7201 aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat
     7261 ccactagtcc agtgtggtgg aattgccctt
//
