LOCUS       sequence-134952-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express in mammalian cells for unnatural amino acid incorporation 
            into VAMP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell
            SW, Lemke EA, Rizzoli SO
  TITLE     Super-resolution Microscopy of Clickable Amino Acids Reveals the 
            Effects of Fluorescent Protein Tagging on Protein Assemblies.
  JOURNAL   ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. 
            Epub 2015 Oct 28.
  PUBMED    26498474
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04434"; journalName: "ACS Nano"; date: 
            "2015-11-24- 24"; volume: "9"; issue: "11"; pages: "11034-41"
COMMENT     Sequence Label: pN1 VAMP2 R125TAG
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    538..659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(575..594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     629..648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(803..824)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctatgt cggctaccgc tgccaccgtc
       61 ccgcctgccg ccccggccgg cgagggtggc ccccctgcac ctcctccaaa tcttactagt
      121 aacaggagac tgcagcagac ccaggcccag gtggatgagg tggtggacat catgagggtg
      181 aatgtggaca aggtcctgga gcgggaccag aagttgtcgg agctggatga ccgtgcagat
      241 gccctccagg caggggcctc ccagtttgaa acaagtgcag ccaagctcaa gcgcaaatac
      301 tggtggaaaa acctcaagat gatgatcatc ttgggagtga tctgcgccat catcctcatc
      361 atcatcatcg tttacttcag cactcgaatt ctgcagtcga cggtaccgta gtaagcccgg
      421 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      481 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
      661 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      721 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      781 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      841 ctccaacgtc aa
//
