LOCUS       sequence-134955-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Theta replicating shuttle vector for E. coli and gram positive 
            bacteria. High copy number in gram positives. Erythromycin 
            resistance, recommended to use BHI agar plates for E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   O'Sullivan DJ, Klaenhammer TR
  TITLE     High- and low-copy-number Lactococcus shuttle cloning vectors with 
            features for clone screening.
  JOURNAL   Gene. 1993 Dec 31;137(2):227-31.
  PUBMED    8299952
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1993-12-31"; volume: "137(2)"; pages: "227-31"
COMMENT     Sequence Label: pTRKH2
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
       61 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      121 aattctattt aatcactttg actagcaaat actaacaaca agacacacac accaaaaatc
      181 aaaaattcac tacttttagt taaaaaccac gtaaccacaa gaactaatcc aatccatgta
      241 atcgggttct tcaaatattt ctccaagatt ttcctcctct aatatgctca acttaaatga
      301 cctattcaat aaatctatta tgctgctaaa tagtttatag gacaaataag tatactctaa
      361 tgacctataa aagatagaaa attaaaaaat caagtgttcg cttcgctctc actgcccctc
      421 gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa gttttcgggc
      481 tactctctcc ttctccccct aataattaat taaaatctta ctctgtatat ttctgctaat
      541 cattcgctaa acagcaaaga aaaaacaaac acgtatcata gatataaatg taatggcata
      601 gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg tgtccgtttt
      661 agggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgctcta agtcaaaaga
      721 atttgtcagc gcctttatat agctttcttt ttcttctttt tttactttaa tgatcgatag
      781 caacaatgat ttaacactag caagttgaat gccaccattt cttcctggtt taatcttaaa
      841 gaa
//
