LOCUS       sequence-134958-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion 
            protein protein. Contains the catalytic domain of mouse Dnmt3a and 
            the C-terminal part of mouse Dnmt3L connected by 27 aa linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Siddique AN, Nunna S, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt 
            R, Rots MG, Ragozin S, Jurkowski TP, Jeltsch A
  TITLE     Targeted methylation and gene silencing of VEGF-A in human cells by 
            using a designed Dnmt3a-Dnmt3L single-chain fusion protein with 
            increased DNA methylation activity.
  JOURNAL   J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 
            10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.
  PUBMED    23220192
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2012.11.038"; journalName: "J Mol Biol"; date: 
            "2013-02-8- 8"; volume: "425"; issue: "3"; pages: "479-91"
COMMENT     Sequence Label: pET28-Dnmt3a3L-sc27
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgaaccat gaccaggaat ttgacccccc aaaggtttac
      121 ccacctgtgc cagctgagaa gaggaagccc atccgcgtgc tgtctctctt tgatgggatt
      181 gctacagggc tcctggtgct gaaggacctg ggcatccaag tggaccgcta cattgcctcc
      241 gaggtgtgtg aggactccat cacggtgggc atggtgcggc accagggaaa gatcatgtac
      301 gtcggggacg tccgcagcgt cacacagaag catatccagg agtggggccc attcgacctg
      361 gtgattggag gcagtccctg caatgacctc tccattgtca accctgcccg caagggactt
      421 tatgagggta ctggccgcct cttctttgag ttctaccgcc tcctgcatga tgcgcggccc
      481 aaggagggag atgatcgccc cttcttctgg ctctttgaga atgtggtggc catgggcgtt
      541 agtgacaaga gggacatctc gcgatttctt gagtctaacc ccgtgatgat tgacgccaaa
      601 gaagtgtctg ctgcacacag ggcccgttac ttctggggta accttcctgg catgaacagg
      661 cctttggcat ccactgtgaa tgataagctg gagctgcaag agtgtctgga gcacggcaga
      721 atagccaagt tcagcaaagt gaggaccatt accaccaggt caaactctat aaagcagggc
      781 aaagaccagc atttccccgt cttcatgaac gagaaggagg acatcctgtg gtgcactgaa
      841 atggaaaggg tgtttggctt c
//
