LOCUS       sequence-134962-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modular shuttle vector for Geobacillus and E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Reeve B, Martinez-Klimova E, de Jonghe J, Leak DJ, Ellis T
  TITLE     The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile 
            Engineering.
  JOURNAL   ACS Synth Biol. 2016 Jul 1.
  PUBMED    27332993
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jul 1."
COMMENT     Sequence Label: pG1AK-mCherry
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(288..307)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     557..575
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgccccttt cggggcgcga aattgagctc ttattaggtg gagtggcggc cctcggcgcg
       61 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt
      121 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      181 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
      241 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      301 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
      361 gtcctgcaag gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
      421 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
      481 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
      541 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      601 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
      661 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      721 tcgaatcact ccttatctag acaatgcttt ttcatcattg cagcgga
//
