LOCUS       sequence-134969-       11004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human dysferlin full length tagged with YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Xu L, Pallikkuth S, Hou Z, Mignery GA, Robia SL, Han R
  TITLE     Dysferlin forms a dimer mediated by the C2 domains and the 
            transmembrane domain in vitro and in living cells.
  JOURNAL   PLoS One. 2011;6(11):e27884. doi: 10.1371/journal.pone.0027884. Epub
            2011 Nov 14.
  PUBMED    22110769
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27884. doi"; pages: " 
            10.1371/journal.pone.0027884. Epub 2011 Nov 14"
COMMENT     Sequence Label: pEYFP-hDF
FEATURES             Location/Qualifiers
     source          1..11004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2056..2067
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7792..7913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7829..7848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7883..7902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7920..8375)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8057..8078)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8269..8288
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8402..8506
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8508..8865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8508..8699
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8531..8551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8716..8851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8900..9694
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8900..9694
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8954..8973)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9564..9583
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9882..9901)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9926..9973
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10302..10890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10791..10810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgatg
     1381 ctgagggtct tcatcctcta tgccgagaac gtccacacac ccgacaccga catcagcgat
     1441 gcctactgct ccgcggtgtt tgcaggggtg aagaagagaa ccaaagtcat caagaacagc
     1501 gtgaaccctg tatggaatga gggatttgaa tgggacctca agggcatccc cctggaccag
     1561 ggctctgagc ttcatgtggt ggtcaaagac catgagacga tggggaggaa caggttcctg
     1621 ggggaagcca aggtcccact ccgagaggtc ctcgccaccc ctagtctgtc cgccagcttc
     1681 aatgcccccc tgctggacac caagaagcag cccacagggg cctcgctggt cctgcaggtg
     1741 tcctacacac cgctgcctgg agctgtgcct ctgttcccgc cccctactcc tctggagccc
     1801 tccccgactc tgcctgacct ggatgtagtg gcagacacag gaggagagga agacacagag
     1861 gaccagggac tcactggaga tgaggcggag ccattcctgg atcaaagcgg aggcccgggg
     1921 gctcccacca ccccaaggaa actaccttca cgtcctccgc cccactaccc cgggatcaaa
     1981 agaaagcgaa gtgcgcctac atctagaaag ctgctgtcag acaaaccgca ggatttccag
     2041 atcagggtcc aggtgatcga ggggcgccag ctgccggggg tgaacatcaa gcctgtggtc
     2101 aaggttaccg ctgcagggca gaccaagcgg acgcggatcc acaagggaaa cagcccactc
     2161 ttcaatgaga ctcttttctt caacttgttt gactctcctg gggagctgtt tgatgagccc
     2221 atctttatca cggtggtaga ctctcgttct ctcaggacag atgctctcct cggggagttc
     2281 cggatggacg tgggcaccat ttacagagag ccccggcacg cctatctcag gaagtggctg
     2341 ctgctctcag accctgatga cttctctgct ggggccagag gctacctgaa aacaagcctt
     2401 tgtgtgctgg ggcctgggga cgaagcgcct ctggagagaa aagacccctc tgaagacaag
     2461 gaggacattg aaagcaacct gctccggccc acaggcgtag ccctgcgagg agcccacttc
     2521 tgcctgaagg tcttccgggc cgaggacttg ccgcagatgg acgatgccgt gatggacaac
     2581 gtgaaacaga tctttggctt cgagagtaac aagaagaact tggtggaccc ctttgtggag
     2641 gtcagctttg cggggaaaat gctgtgcagc aagatcttgg agaagacggc caaccctcag
     2701 tggaaccaga acatcacact gcctgccatg tttccctcca tgtgcgaaaa aatgaggatt
     2761 cgtatcatag actgggaccg cctgactcac aatgacatcg tggctaccac ctacctgagt
     2821 atgtcgaaaa tctctgcccc tggaggagaa atagaagagg agcctgcagg tgctgtcaag
     2881 ccttcgaaag cctcagactt ggatgactac ctgggcttcc tccccacttt tgggccctgc
     2941 tacatcaacc tctatggcag tcccagagag ttcacaggct tcccagaccc ctacacagag
     3001 ctcaacacag gcaaggggga aggtgtggct tatcgtggcc ggcttctgct ctccctggag
     3061 accaagctgg tggagcacag tgaacagaag gtggaggacc ttcctgcgga tgacatcctc
     3121 cgggtggaga agtaccttag gaggcgcaag tactccctgt ttgcggcctt ctactcagcc
     3181 accatgctgc aggatgtgga tgatgccatc cagtttgagg tcagcatcgg gaactacggg
     3241 aacaagttcg acatgacctg cctgccgctg gcctccacca ctcagtacag ccgtgcagtc
     3301 tttgacgggt gccactacta ctacctaccc tggggtaacg tgaaacctgt ggtggtgctg
     3361 tcatcctact gggaggacat cagccataga atcgagactc agaaccagct gcttgggatt
     3421 gctgaccggc tggaagctgg cctggagcag gtccacctgg ccctgaaggc gcagtgctcc
     3481 acggaggacg tggactcgct ggtggctcag ctgacggatg agctcatcgc aggctgcagc
     3541 cagcctctgg gtgacatcca tgagacaccc tctgccaccc acctggacca gtacctgtac
     3601 cagctgcgca cccatcacct gagccaaatc actgaggctg ccctggccct gaagctcggc
     3661 cacagtgagc tccctgcagc tctggagcag gcggaggact ggctcctgcg tctgcgtgcc
     3721 ctggcagagg agccccagaa cagcctgccg gacatcgtca tctggatgct gcagggagac
     3781 aagcgtgtgg cataccagcg ggtgcccgcc caccaagtcc tcttctcccg gcggggtgcc
     3841 aactactgtg gcaagaattg tgggaagcta cagacaatct ttctgaaata tccgatggag
     3901 aaggtgcctg gcgcccggat gccagtgcag atacgggtca agctgtggtt tgggctctct
     3961 gtggatgaga aggagttcaa ccagtttgct gaggggaagc tgtctgtctt tgctgaaacc
     4021 tatgagaacg agactaagtt ggcccttgtt gggaactggg gcacaacggg cctcacctac
     4081 cccaagtttt ctgacgtcac gggcaagatc aagctaccca aggacagctt ccgcccctcg
     4141 gccggctgga cctgggctgg agattggttc gtgtgtccgg agaagactct gctccatgac
     4201 atggacgccg gtcacctgag cttcgtggaa gaggtgtttg agaaccagac ccggcttccc
     4261 ggaggccagt ggatctacat gagtgacaac tacaccgatg tgaacgggga gaaggtgctt
     4321 cccaaggatg acattgagtg cccactgggc tggaagtggg aagatgagga atggtccaca
     4381 gacctcaacc gggctgtcga tgagcaaggc tgggagtata gcatcaccat ccccccggag
     4441 cggaagccga agcactgggt ccctgctgag aagatgtact acacacaccg acggcggcgc
     4501 tgggtgcgcc tgcgcaggag ggatctcagc caaatggaag cactgaaaag gcacaggcag
     4561 gcggaggcgg agggcgaggg ctgggagtac gcctctcttt ttggctggaa gttccacctc
     4621 gagtaccgca agacagatgc cttccgccgc cgccgctggc gccgtcgcat ggagccactg
     4681 gagaagacgg ggcctgcagc tgtgtttgcc cttgaggggg ccctgggcgg cgtgatggat
     4741 gacaagagtg aagattccat gtccgtctcc accttgagct tcggtgtgaa cagacccacg
     4801 atttcctgca tattcgacta tgggaaccgc taccatctac gctgctacat gtaccaggcc
     4861 cgggacctgg ctgcgatgga caaggactct ttttctgatc cctatgccat cgtctccttc
     4921 ctgcaccaga gccagaagac ggtggtggtg aagaacaccc ttaaccccac ctgggaccag
     4981 acgctcatct tctacgagat cgagatcttt ggcgagccgg ccacagttgc tgagcaaccg
     5041 cccagcattg tggtggagct gtacgaccat gacacttatg gtgcagacga gtttatgggt
     5101 cgctgcatct gtcaaccgag tctggaacgg atgccacggc tggcctggtt cccactgacg
     5161 aggggcagcc agccgtcggg ggagctgctg gcctcttttg agctcatcca gagagagaag
     5221 ccggccatcc accatattcc tggttttgag gtgcaggaga catcaaggat cctggatgag
     5281 tctgaggaca cagacctgcc ctacccacca ccccagaggg aggccaacat ctacatggtt
     5341 cctcagaaca tcaagccagc gctccagcgt accgccatcg agatcctggc atggggcctg
     5401 cggaacatga agagttacca gctggccaac atctcctccc ccagcctcgt ggtagagtgt
     5461 gggggccaga cggtgcagtc ctgtgtcatc aggaacctcc ggaagaaccc caactttgac
     5521 atctgcaccc tcttcatgga agtgatgctg cccagggagg agctctactg cccccccatc
     5581 accgtcaagg tcatcgataa ccgccagttt ggccgccggc ctgtggtggg ccagtgtacc
     5641 atccgctccc tggagagctt cctgtgtgac ccctactcgg cggagagtcc atccccacag
     5701 ggtggcccag acgatgtgag cctactcagt cctggggaag acgtgctcat cgacattgat
     5761 gacaaggagc ccctcatccc catccaggag gaagagttca tcgattggtg gagcaaattc
     5821 tttgcctcca taggggagag ggaaaagtgc ggctcctacc tggagaagga ttttgacacc
     5881 ctgaaggtct atgacacaca gctggagaat gtggaggcct ttgagggcct gtctgacttt
     5941 tgtaacacct tcaagctgta ccggggcaag acgcaggagg agacagaaga tccatctgtg
     6001 attggtgaat ttaagggcct cttcaaaatt tatcccctcc cagaagaccc agccatcccc
     6061 atgcccccaa gacagttcca ccagctggcc gcccagggac cccaggagtg cttggtccgt
     6121 atctacattg tccgagcatt tggcctgcag cccaaggacc ccaatggaaa gtgtgatcct
     6181 tacatcaaga tctccatagg gaagaaatca gtgagtgacc aggataacta catcccctgc
     6241 acgctggagc ccgtatttgg aaagatgttc gagctgacct gcactctgcc tctggagaag
     6301 gacctaaaga tcactctcta tgactatgac ctcctctcca aggacgaaaa gatcggtgag
     6361 acggtcgtcg acctggagaa caggctgctg tccaagtttg gggctcgctg tggactccca
     6421 cagacctact gtgtctctgg accgaaccag tggcgggacc agctccgccc ctcccagctc
     6481 ctccacctct tctgccagca gcatagagtc aaggcacctg tgtaccggac agaccgtgta
     6541 atgtttcagg ataaagaata ttccattgaa gagatagagg ctggcaggat cccaaaccca
     6601 cacctgggcc cagtggagga gcgtctggct ctgcatgtgc ttcagcagca gggcctggtc
     6661 ccggagcacg tggagtcacg gcccctctac agccccctgc agccagacat cgagcagggg
     6721 aagctgcaga tgtgggtcga cctatttccg aaggccctgg ggcggcctgg acctcccttc
     6781 aacatcaccc cacggagagc cagaaggttt ttcctgcgtt gtattatctg gaataccaga
     6841 gatgtgatcc tggatgacct gagcctcacg ggggagaaga tgagcgacat ttatgtgaaa
     6901 ggttggatga ttggctttga agaacacaag caaaagacag acgtgcatta tcgttccctg
     6961 ggaggtgaag gcaacttcaa ctggaggttc attttcccct tcgactacct gccagctgag
     7021 caagtctgta ccattgccaa gaaggatgcc ttctggaggc tggacaagac tgagagcaaa
     7081 atcccagcac gagtggtgtt ccagatctgg gacaatgaca agttctcctt tgatgatttt
     7141 ctgggctccc tgcagctcga tctcaaccgc atgcccaagc cagccaagac agccaagaag
     7201 tgctccttgg accagctgga tgatgctttc caccccgaat ggtttgtgtc cctttttgag
     7261 cagaaaacag tgaagggctg gtggccctgt gtagcagaag agggtgagaa gaaaatactg
     7321 gcgggcaagc tggaaatgac cttggagatt gtagcagaga gtgagcatga ggagcggcct
     7381 gctggccagg gccgggatga gcccaacatg aaccctaagc ttgaggaccc aaggcgcccc
     7441 gacacctcct tcctgtggtt tacctcccca tacaagacca tgaagttcat cctgtggcgg
     7501 cgtttccggt gggccatcat cctcttcatc atcctcttca tcctgctgct gttcctggcc
     7561 atcttcatct acgccttccc gaactatgct gccatgaagc tggtgaagcc cttcagctga
     7621 ggactctcct gccctgtaga aggggccggt accgcgggcc cgggatccac cggatctaga
     7681 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     7741 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     7801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     7921 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7981 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8041 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8101 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     8161 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     8221 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8281 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8341 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     8401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8461 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     8521 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8581 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8701 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8761 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8821 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     8881 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     8941 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     9001 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     9061 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     9121 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     9181 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     9241 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     9301 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     9361 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     9421 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     9481 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     9541 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     9601 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     9661 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     9721 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     9781 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     9841 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     9901 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     9961 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
    10021 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
    10081 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
    10141 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10201 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10441 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10681 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10921 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10981 gataaccgta ttaccgccat gcat
//
