LOCUS       sequence-134983-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express FLPe, copGFP and Puromycin resistance gene under CB 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CB-FLPe-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(305..325)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          409..505
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 attcccaagc aggaattaat ttctttaatg aggcttccag aattgttgct ttttgcgtct
       61 tgtatttaaa ctggagtgat ttgttgacaa tatcgaaact cagcgaattg cttatgatag
      121 tattatagct catgaatgtg gctctcttga ttgctgttcc gttatgagta atcatccaac
      181 ataaataggt tagttcagca gcacatgatg ctattttttc ccctgaaggt ctttcaaacc
      241 tttccacaaa ctgacgaacc aggaccttag gtggtgtttt acataatata tcaaattgac
      301 tcatcacctt cctcttcttc ttgggagcca tggtggatcc cgaccggtgg atcggccgcg
      361 ggtacaattc cgcagctttt agagcagaag taacacttcc gtacaggcct agaagtaaag
      421 gcaacatcca ctgaggagca gttctttgat ttgcaccacc accggatcgg ggacctgaaa
      481 taaaagacaa aaagactaaa cttaccagtt aactttctgg tttttcagtt cctcgatcga
      541 ctctagggcc gccaccgcgg tggctgatcc cgctcccgcc cgccgcgcgc ttcgcttttt
      601 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
      661 attggctgcc gccgcacctc tccgcctcgc c
//
