LOCUS       sequence-134992-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gene of interest under truncated EF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     express gene of interest under truncated EF-1 promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1s
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             84..352
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(365..576)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    617..733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcgcg gccgcgttta aacgctagcg gatccgtcta
       61 gcgtaggcgc cggtcacagc ttggatctgt aacggcgcag aacagaaaac gaaacaaaga
      121 cgtagagttg agcaagcagg gtcaggcaaa gcgtggagag ccggctgagt ctaggtaggc
      181 tccaagggag cgccggacaa aggcccggtc tcgacctgag ctttaaactt acctagacgg
      241 cggacgcagt tcaggaggca ccacaggcgg gaggcggcag aacgcgactc aaccggcgtg
      301 gatggcggcc tcaggtaggg cggcgggcgc gtgaaggaga gatgcgagcc cctcgaagct
      361 tcagctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac
      421 ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt
      481 cccagtttac cccgcgccac cttctctagg cacccgttca attgccgacc cctcccccca
      541 acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggcaccg gagcgatcgc
      601 agatccttgt atcgataaaa ttttgaattt tgtaatttgt ttttgtaatt ctttagtttg
      661 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
      721 ttttctttta aaagttaacc gataccgtcg agatccgttc actaatcgaa tggatctgtc
      781 tctgtctctc tctccacctt cttcttctat tccttcgg
//
