LOCUS       sequence-134993-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gene of interest under truncated EF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     express gene of interest under truncated EF-1 promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1s
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             8..276
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     complement(398..418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
       61 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
      121 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
      181 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
      241 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
      301 agacggatcc gctagcgttt aaacgcggcc gcgaattcgt cgacaatcaa cctctggatt
      361 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
      421 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
      481 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
      541 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
      601 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
      661 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
      721 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
      781 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctca
//
