LOCUS       sequence-135000-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human dysferlin C2B domain tagged with YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Xu L, Pallikkuth S, Hou Z, Mignery GA, Robia SL, Han R
  TITLE     Dysferlin forms a dimer mediated by the C2 domains and the 
            transmembrane domain in vitro and in living cells.
  JOURNAL   PLoS One. 2011;6(11):e27884. doi: 10.1371/journal.pone.0027884. Epub
            2011 Nov 14.
  PUBMED    22110769
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27884. doi"; pages: " 
            10.1371/journal.pone.0027884. Epub 2011 Nov 14"
COMMENT     Sequence Label: pEYFP-hDF C2B
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(355..366)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(403..654)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(445..466)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggtacccct tacagcacac aaaggcttgt tttcaggtag cctctggccc cagcagagaa
       61 gtcatcaggg tctgagagca gcagccactt cctgagatag gcatgccggg gctctctgta
      121 aatggtgccc acgtccatcc ggaactcccc gaggagagca tctgtcctga gagaacgaga
      181 gtctaccacc gtgataaaga tgggctcatc aaacagctcc ccaggagagt caaacaagtt
      241 gaagaaaaga gtctcattga agagtgggct gtttcccttg tggatccgcg tccgcttggt
      301 ctgccctgca gcggtaacct tgaccacagg cttgatgttc acccccggca gctggcgccc
      361 ctcgatcacc tggaccctga tctgacgctc gagatctgag tacttgtaca gctcgtccat
      421 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      481 gttggggtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg gcagcagcac
      541 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      601 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      661 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      721 ctccttgaag tcgatgccct tcagctcgat gcgg
//
