LOCUS       sequence-135010-        4442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with 
            c-myc tag and His6-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK137
FEATURES             Location/Qualifiers
     source          1..4442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             2125..2154
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2170..2187
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2266..2512
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2527..2938
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2946..2993
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3012..3386
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3452..3699
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3468..3486)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(3774..4362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3854..3873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggcccagc
      961 cggccgtctc ggatcggtac ctcgagatcc atgggtggtg gcggccgcgg tagaacttct
     1021 caaaaaccgt tgatggagcg tgtttctgtc gacccgccgt tcaccgtgtc tgacctgaaa
     1081 caagctatcc cgccacactg tttcaagcgt tctgtgatca gatcttctta ctacatcgta
     1141 cacgacgcga taatcgctta cattttctac ttcctggctg acaaatacat ccctattctg
     1201 ccagccccgc tagcctacct agcttggccg ctgtattggt tctgtcaagc ttctatcctg
     1261 accggtctgt gggtaatcgg ccacgagtgc ggtcaccacg cgttctctga ctaccagtgg
     1321 gtagacgaca ccgttggttt cattttgcac tctttcctaa tgactccata cttcagctgg
     1381 aaatactctc accgtaacca ccacgctaac acgaattctc tggacaacga tgaggtttac
     1441 atcccgaaat ctaaagccaa agtagctctg tactataaag ttctgaacca ccccccgggt
     1501 cgtttattaa tcatgttcat caccttcact ttaggtttcc cattatacct cttcaccaac
     1561 atctctggta aaaaatacga gcgtttcgct aaccacttcg atccgatgag tccaatcttc
     1621 aaggagagag agagattcca ggttctgctg tccgacctag gtctactagc tgttttatac
     1681 ggtgttaaat tggctgttgc tgctaagggt gccgcttggg taacctgcat ctatgggatc
     1741 ccggttctag gcgtgttcat cttcttcgat atcatcactt acctgcacca cacccacctg
     1801 tccttgccac actacgactc ttctgagtgg aactggctgc gtggagctct atctaccatc
     1861 gacagagact tcggtttcct gaactctgtt ctgcacgatg ttacccacac ccacgtaatg
     1921 catcacttgt tcagctacat cccgcactac cacgctaagg aggctagaga cgctatcaac
     1981 actgttctgg gcgacttcta caaaatcgat agaactccaa tattgaaagc catgtggcgt
     2041 gaggctaagg agtgcatctt catcgagcca gagaaaggca gagagtctaa aggtgtttac
     2101 tggtacaaca aattcgcggg gcccgaacaa aaactcatct cagaagagga tctgaatagc
     2161 gccgtcgacc atcatcatca tcatcattga gttttagcct tagacatgac tgttcctcag
     2221 ttcaagttgg gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag
     2281 aatgccattt gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat
     2341 atagtatagg attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc
     2401 ctatctcgca gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat
     2461 gtttttcttg gtatttccca ctcctcttca gagtacagaa gattaagtga gaccttcgtt
     2521 tgtgcggatc ccccacacac catagcttca aaatgtttct actccttttt tactcttcca
     2581 gattttctcg gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta
     2641 aattttccct ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa
     2701 gaaaaaagag accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc
     2761 acgtttcttt ttcttgaaat tttttttttt agtttttttc tctttcagtg acctccattg
     2821 atatttaagt taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct
     2881 attacaactt tttttacttc ttgttcatta gaaagaaagc atagcaatct aatctaaggg
     2941 gcggtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     3001 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     3061 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     3121 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     3181 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     3241 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     3301 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     3361 acttcgtggc cgaggagcag gactgacacg tccgacggcg gcccacgggt cccaggcctc
     3421 ggagatccgt cccccttttc ctttgtcgat atcatgtaat tagttatgtc acgcttacat
     3481 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     3541 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     3601 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     3661 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca agctggagac caacatgtga
     3721 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3781 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3961 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4021 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4081 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4141 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4201 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4261 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4321 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4381 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4441 tc
//
