LOCUS       sequence-135013-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid to express destabilized GFP under the control of 
            the ATF-responsive CHOP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, 
            Zgraggen E, Salmon P, Kiss JZ
  TITLE     Wnt signaling regulates multipolar-to-bipolar transition of 
            migrating neurons in the cerebral cortex.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 
            10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26.
  PUBMED    25732825
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.061"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1349-61"
COMMENT     Sequence Label: pCLX-CHOP-dGFP
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(82..204,205..210,211..921,922..924,
                     925..927))
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(253..274)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     602..621
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     861..882
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(933..957)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgatta tcggaattcg attatcacca
       61 ctttgtacaa gaaagctggg tctacacatt gatcctagca gaagcacagg ctgcagggtg
      121 acggtccatc ccgctctcct gggcacaaga catgggcagc gtgccatcat cctgctcctc
      181 cacctccggc gggaagccat ggctaagctt cttgtacagc tcgtccatgc cgagagtgat
      241 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      301 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      361 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      421 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
      481 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      541 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      601 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      661 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
      721 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      781 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      841 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      901 gaacagctcc tcgcccttgc tcaccatggt gaagcctgct tttttgtaca aacttgtctc
      961 ctcaggttcc agctctgatt ttggctctgt cgctgccacc cgctcatctt taacatgata
     1021 cgctcagtgc ctta
//
