LOCUS       sequence-135021-        6331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mitochondrial unfolded protein response research.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Zhao Q, Wang J, Levichkin IV, Stasinopoulos S, Ryan MT, Hoogenraad 
            NJ
  TITLE     A mitochondrial specific stress response in mammalian cells.
  JOURNAL   EMBO J. 2002 Sep 2;21(17):4411-9.
  PUBMED    12198143
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2002-09-2"; volume: "21(17)"; pages: "4411-9"
COMMENT     Sequence Label: Ornithine transcarbamylase (OTC)
FEATURES             Location/Qualifiers
     source          1..6331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3355..3374)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3420..3475
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3474..3493)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3834..3850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3834..3850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3847..3869)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3858..3874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3882..3888,3889..3906,3907..3912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3927..3948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4007..4202
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4053..4188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4208..4342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4245..4264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4299..4318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4410..4427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4581..5169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4661..4680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5340..6131,6132..6200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5963..5982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6201..6305)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggatc ccctctgcaa
     1741 ctgaaagcat tcttagcttg ccagtggccc ccactgcctg cctgcctgcg gaactctcta
     1801 gaccatagat tcctcctcca ctctagcaag agaagatgct gtctaatttg aggatcctgc
     1861 tcaacaaggc agctcttaga aaggctcaca cttccatggt tcgaaatttt cggtatggga
     1921 agccagtcca gagtcaagta cagctgaaag gccgtgacct cctcaccctg aagaacttca
     1981 caggagagga gattcagtac atgctatggc tctctgcaga tctgaaattc aggatcaaac
     2041 agaaaggaga atacttgcct ttattgcaag ggaaatcctt agggatgatt tttgagaaaa
     2101 gaagtactcg aacaagactg tccacagaaa caggcttcgc tcttctggga ggacatcctt
     2161 cttttcttac cacacaagac attcacttgg gcgtgaatga aagtctcaca gacacagctc
     2221 gtgtgttatc tagcatgaca gatgcagtgt tagctcgagt gtataaacaa tcagatctgg
     2281 acatcctggc taaggaagca accatcccaa ttgtcaacgg actgtcagac ctgtatcatc
     2341 ctatccagat cctggctgat taccttacac tccaggaaca ctatggctct ctcaaaggtc
     2401 tcaccctcag ctggatagga gatgggaaca atatcctgca ctccatcatg atgagtgctg
     2461 caaaattcgg gatgcacctt caagcagcta ctccaaaggg ttatgagcca gatcctaata
     2521 tagtcaagct agcagagcag tatgccaagg agaatggtac caggttgtca atgacaaatg
     2581 atccactgga agcagcacgt ggaggcaatg tattaattac agatacttgg ataagcatgg
     2641 gacaagagga tgagaagaaa aagcgtcttc aagctttcca aggttaccag gttacaatga
     2701 agactgctaa agtggctgcg tctgactgga cgtttttaca ctgcttgcct agaaagccag
     2761 aagaagtaga tgatgaagtg ttttattctc cgcggtcatt agtgttccca gaggcagaaa
     2821 atagaaagtg gacaatcatg gctgtcatgg tatccctgct gacagactac tcacctgtgc
     2881 tccagaagcc aaagttctga tgcctgtcaa gaggacgaaa aacccaaaag acaaaaaaat
     2941 ctgttcttta gcagcagaat aagtcagttt atgtagaaaa gagaagaatt gaaattgtaa
     3001 acacatccct agtgcgtgat ataattatgt aattgctttg ctattgtgag aattgcttaa
     3061 agcttttagt ttaagtgctg ggcattttat tatcctgctt gacttgactt aagcactctc
     3121 ttcaattcac aacttctgaa tgatatttgg gtttcatatt aattatcata cacatttcct
     3181 tccactaagc attaaacact atgcttacaa tgcataccat ctaagtcatt aaatgtaatc
     3241 catgcttatt accttaaaaa aaaaaaaacc cgaattcact cctcaggtgc aggctgccta
     3301 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     3361 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     3421 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     3481 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     3541 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt
     3601 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     3661 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     3721 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     3781 ctcttctctt atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     3841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4201 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4321 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4741 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6301 ccgcgcacat ttccccgaaa agtgccacct g
//
