LOCUS       sequence-135024-        4617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with 
            Pas2p signal sequence, c-myc tag, and His6-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK141
FEATURES             Location/Qualifiers
     source          1..4617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             2300..2329
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2345..2362
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2441..2687
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2702..3113
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3121..3168
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3187..3561
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3627..3874
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3643..3661)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(3949..4537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4029..4048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggatcatc
      961 attgcaaagc cataaactct gagaggaaca caagtgcata caatcactag atatttgcaa
     1021 agtcgtaatg ttggagtaca cggcaggatt aataagacga aacaagaaaa agtttcttat
     1081 atcctctgga atcataggag taggttacta tgtaaccaaa actatcaata acaagatcca
     1141 agaattccaa aaccgctcga gatccatggg tggtggcggc cgcggtagaa cttctcaaaa
     1201 accgttgatg gagcgtgttt ctgtcgaccc gccgttcacc gtgtctgacc tgaaacaagc
     1261 tatcccgcca cactgtttca agcgttctgt gatcagatct tcttactaca tcgtacacga
     1321 cgcgataatc gcttacattt tctacttcct ggctgacaaa tacatcccta ttctgccagc
     1381 cccgctagcc tacctagctt ggccgctgta ttggttctgt caagcttcta tcctgaccgg
     1441 tctgtgggta atcggccacg agtgcggtca ccacgcgttc tctgactacc agtgggtaga
     1501 cgacaccgtt ggtttcattt tgcactcttt cctaatgact ccatacttca gctggaaata
     1561 ctctcaccgt aaccaccacg ctaacacgaa ttctctggac aacgatgagg tttacatccc
     1621 gaaatctaaa gccaaagtag ctctgtacta taaagttctg aaccaccccc cgggtcgttt
     1681 attaatcatg ttcatcacct tcactttagg tttcccatta tacctcttca ccaacatctc
     1741 tggtaaaaaa tacgagcgtt tcgctaacca cttcgatccg atgagtccaa tcttcaagga
     1801 gagagagaga ttccaggttc tgctgtccga cctaggtcta ctagctgttt tatacggtgt
     1861 taaattggct gttgctgcta agggtgccgc ttgggtaacc tgcatctatg ggatcccggt
     1921 tctaggcgtg ttcatcttct tcgatatcat cacttacctg caccacaccc acctgtcctt
     1981 gccacactac gactcttctg agtggaactg gctgcgtgga gctctatcta ccatcgacag
     2041 agacttcggt ttcctgaact ctgttctgca cgatgttacc cacacccacg taatgcatca
     2101 cttgttcagc tacatcccgc actaccacgc taaggaggct agagacgcta tcaacactgt
     2161 tctgggcgac ttctacaaaa tcgatagaac tccaatattg aaagccatgt ggcgtgaggc
     2221 taaggagtgc atcttcatcg agccagagaa aggcagagag tctaaaggtg tttactggta
     2281 caacaaattc gcggggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt
     2341 cgaccatcat catcatcatc attgagtttt agccttagac atgactgttc ctcagttcaa
     2401 gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc
     2461 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt
     2521 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc
     2581 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt
     2641 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct tcgtttgtgc
     2701 ggatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt
     2761 tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt
     2821 tccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa
     2881 aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt
     2941 tctttttctt gaaatttttt tttttagttt ttttctcttt cagtgacctc cattgatatt
     3001 taagttaata aacggtcttc aatttctcaa gtttcagttt catttttctt gttctattac
     3061 aacttttttt acttcttgtt cattagaaag aaagcatagc aatctaatct aaggggcggt
     3121 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     3181 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     3241 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     3301 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     3361 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     3421 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     3481 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     3541 gtggccgagg agcaggactg acacgtccga cggcggccca cgggtcccag gcctcggaga
     3601 tccgtccccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg
     3661 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     3721 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     3781 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     3841 gaaggttttg ggacgctcga aggctttaat ttgcaagctg gagaccaaca tgtgagcaaa
     3901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4141 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4381 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagatc
//
