LOCUS       sequence-135038-        5650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes isoform 2 of Cep123 (Cep89/CCDC123) fused to a myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Sillibourne JE, Hurbain I, Grand-Perret T, Goud B, Tran P, Bornens M
  TITLE     Primary ciliogenesis requires the distal appendage component Cep123.
  JOURNAL   Biol Open. 2013 Apr 9;2(6):535-45. doi: 10.1242/bio.20134457. Print 
            2013 Jun 15.
  PUBMED    23789104
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/bio.20134457"; journalName: "Biol Open"; date: "2013-04-9- 
            9"; volume: "2"; issue: "6"; pages: "535-45"
COMMENT     Sequence Label: pMyc-C1-Cep123 (isoform 2)
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..33
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1828..1949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1865..1884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1919..1938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2093..2114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2305..2324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2438..2542
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2544..2901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2544..2735
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2567..2587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2752..2887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2936..3730
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2936..3730
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2990..3009)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3600..3619
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3918..3937)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3962..4009
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4338..4926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4827..4846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5101..5404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5405..5608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5559..5579
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggaacaaa aacttatttc tgaagaagat ctgctgtaca agtccggact cagatctcga
       61 gctcaagctt cgaattctgc agtcgacatg gacctaaaca atatgaatca aagccttacc
      121 cttgaactaa acacaatgaa acaagcaatg aaagaactac agttaaaact taagggaatg
      181 gaaaaagaga agagaaagct caaagaggct gagaaggcgt cgtcacagga agttgctgca
      241 cctgaattac tttatctgcg aaaacaagct caagaactgg tggatgaaaa tgatggattg
      301 aaaatgactg tccatcgttt gaatgtagaa ctcagtcgat atcagacaaa attcaggcat
      361 ttgtccaagg aagagagctt aaatattgaa ggcctcccat ccaagggccc tataccaccc
      421 tggttgttgg atataaagta cctgtcacca ttgttgctgg cttatgaaga tatgatgaaa
      481 gagaaggacg agctcaatgc caccctcaag gaggaaatga gaatgtttag gatgcgagtc
      541 caagaagtgg tgaaagaaaa tgaagaattg caccaagagt taaataagag tagtgctgtt
      601 accagtgagg aatggcgtca gcttcagact caagcaaaac tggttttaga ggaaaacaag
      661 ttgttgctgg agcagttgga gattcagcaa aggaaagcca aggacagcca ccaggagcgc
      721 ctccaagaag tttctaagct gactaaacaa ctaatgctcc tggaggcaaa aacccacggc
      781 caggaaaagg agctggcgga gaacagggaa cagctggaga ttttacgtgc caaatgccaa
      841 gaactcaaaa cacactcgga tggcaaaatc gcagtggaag ttcataaatc aattgtgaat
      901 gaattaaaaa gccaattaca gaaggaagaa gagaaagaaa gggctgagat ggaggagttg
      961 atggagaagc tgacagtcct gcaagcgcag aagaagagcc tgctgttaga gaagaacagt
     1021 ttgacagagc aaaacaaagc actggaagcc gaacttgaac gagcacagaa aatcaatagg
     1081 aaatctcaaa agaaaattga ggtcctcaaa aagcaggtgg aaaaagccat ggggaacgaa
     1141 atgtctgctc atcagtacct ggcaaacctt gttggcctgg cagaaaatat aacccaggaa
     1201 cgtgacagtc ttatgtgttt ggcaaaatgt ttagaaagtg agaaggatgg agtgcttaat
     1261 aaagtcataa aaagcaacat tcgcctggga aagttagagg aaaaagtcaa gggctacaag
     1321 aagcaggcag cactgaagct gggggacatc agtcaccgtc tgctggagca gcaggaggac
     1381 ttcgccggca agacagccca gtaccggcag gagatgcggc acctgcacca ggtgctgaag
     1441 gacaagcagg aggtgctgga ccaggcgctg cagcagaaca gagaaatgga aggtgaactt
     1501 gaagttattt gggaatctac cttcagggaa aaccgaagaa tccgagaact tctccaggac
     1561 acactcacga ggacaggcgt gcaggacaac cccagagctc tggttgcccc cagcctcaat
     1621 ggcgtctctc aggcagacct gctggacggc tgcgatgtct gctcctatga cctgaagtct
     1681 catgccccca cctgctaggg atccaccgga tctagataac tgatcataat cagccatacc
     1741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     1801 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     1861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1921 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2401 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2521 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     2581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2821 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2881 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     2941 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3001 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3061 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     3121 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3181 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3241 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3301 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3361 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3421 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     3481 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3541 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3601 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3661 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3721 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     3781 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     3841 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3901 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     3961 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     4021 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4081 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4141 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     4201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4981 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     5041 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     5101 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     5161 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     5221 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     5281 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     5341 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     5401 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     5461 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     5521 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     5581 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     5641 ccggtccacc
//
