LOCUS       sequence-135049-        9332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct expressing the upper MVA pathway in Synechocystis PCC 
            6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9332)
  TITLE     Expression of the MVA pathway in cyanobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PsbA2-HMGS-HMGR-ATOB-NPTI
FEATURES             Location/Qualifiers
     source          1..9332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5769..6584
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7111..7127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8272..9063,9064..9132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8895..8914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9133..9237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9305..9323
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctta attaaacgat
      421 tgcggcttta gcgttccagt ggatatttgc tgggggttaa tgaaacattg tggcggaacc
      481 cagggacaat gtgaccaaaa aattcaggga tatcaataag tattaggtat atggatcata
      541 attgtatgcc cgactattgc ttaaactgac tgaccactga ccttaagagt aatggcgtgc
      601 aaggcccagt gatcaatttc attatttttc attatttcat ctccattgtc cctgaaaatc
      661 agttgtgtcg cccctctaca cagcccagaa ctatggtaaa ggcgcacgaa aaaccgccag
      721 gtaaactctt ctcaaccccc aaaacgccct ctgtttaccc atggaaaaaa cgacaattac
      781 aagaaagtaa aacttatgtc atctataagc ttcgtgtata ttaacttcct gttacaaagc
      841 tttacaaaac tctcattaat cctttagact aagtttagtc agttccaatc tgaacatcga
      901 caaatacata aggaattaat taaatgacta ttggaattga caaaatctcc ttttttgttc
      961 ctccttatta tattgatatg acagccctag ctgaagcacg taatgtggat cccggcaaat
     1021 tccatattgg tatcgggcag gatcaaatgg ctgtcaaccc aattagtcaa gatatcgtta
     1081 cttttgctgc taatgctgcg gaagctatct taacgaaaga agataaagaa gccattgaca
     1141 tggtcattgt gggcacggaa tcctctatcg atgaatccaa agctgctgcc gtcgttctac
     1201 atcggctcat ggggattcaa cctttcgccc ggagctttga gatcaaggaa gcctgctatg
     1261 gagccaccgc cggtttgcag ttggccaaaa atcatgtggc gctccacccg gataaaaagg
     1321 tgttggtggt agccgccgat attgccaaat acggattaaa tagtggcggg gaacccactc
     1381 aaggggcggg cgccgttgcc atgctcgtgg cctccgagcc ccggatcctg gcgttaaaag
     1441 aagataatgt gatgctgact caagacattt atgatttctg gcgccccacc ggccacccgt
     1501 atcctatggt tgatggaccc ctgtctaatg aaacctacat ccaaagcttt gcgcaggtgt
     1561 gggatgaaca taaaaaacgg acaggattag actttgcgga ctacgacgcc ttagcttttc
     1621 acattccgta caccaagatg ggcaagaaag ccttactggc caaaatctcc gatcagacgg
     1681 aagccgaaca agaacgcatc ctggcacggt atgaggaaag tatcgtgtac agtcgccgcg
     1741 tggggaattt atatactgga tccttatact tgggcttaat tagcctgctg gaaaatgcca
     1801 cgaccctgac cgcaggtaat cagatcggct tgttcagtta cgggtctggg gccgtggctg
     1861 aattttttac cggggagttg gtcgccggtt accaaaatca tctccagaag gagacccatt
     1921 tggccttgct agataaccgc acggaattaa gtattgccga gtatgaagcc atgtttgccg
     1981 agacgctcga tactgatatc gatcaaacct tagaagacga attgaagtat agcatcagtg
     2041 cgattaacaa taccgtacgg tcctaccgta attgaggaat taggaggtaa tatatgaaaa
     2101 cggtcgtgat tattgatgcg ctgcgcacac ctattggcaa gtataagggt agtttaagcc
     2161 aagtatccgc tgtggatctg ggtactcacg tgacgacaca actgttgaag cggcacagca
     2221 ccatttctga agagatcgat caagtcattt ttggtaatgt tttgcaggct ggcaatggcc
     2281 agaaccccgc tcggcagatt gccattaaca gtggtttgag tcacgaaatc cctgctatga
     2341 ccgtgaacga agtttgcgga tccggtatga aagccgtgat cctcgccaag caattgatcc
     2401 aattgggtga agccgaagtt ttaattgccg gcggcattga aaatatgtcc caagccccca
     2461 aattacaacg ctttaattat gagaccgaaa gctacgacgc ccctttcagt tctatgatgt
     2521 atgacggcct caccgacgca tttagtggcc aggctatggg gttaacggcc gagaatgtgg
     2581 ccgagaaata tcatgtgacc cgggaagagc aggatcaatt tagcgtgcat agtcaattaa
     2641 aggcggccca agcccaggcg gaaggcattt ttgccgatga aattgctccc ttggaggtca
     2701 gcggtacatt ggtagagaag gatgagggaa ttcggcccaa ttccagcgtt gaaaaattgg
     2761 gaaccttgaa aaccgttttc aaagaagatg ggacggttac cgccggtaac gcgagtacga
     2821 tcaatgatgg cgcaagtgca ctgattattg cgagccagga gtatgccgaa gcccatggtc
     2881 tcccctatct ggcaatcatt cgtgactctg ttgaggttgg gattgatccg gcgtacatgg
     2941 gtattagtcc cattaaagcg atccagaaat tgctggcacg taatcagctg acgaccgagg
     3001 aaattgacct ctatgaaatt aatgaagcct ttgctgccac ctctattgtg gtccaacggg
     3061 aattggcttt acccgaggaa aaggtgaata tttatggtgg aggcattagc ctaggtcacg
     3121 ccattggtgc gacaggagcg cgcctgttaa cgtctctgag ttaccaatta aaccaaaagg
     3181 agaaaaaata tggggtagcc agtctgtgta ttggtggagg cttaggactg gccatgttgt
     3241 tggaacgtcc ccaacaaaag aaaaactctc ggttctacca aatgtcccct gaggagcggc
     3301 tggcgtcctt gctaaatgaa ggacagatca gtgctgacac caaaaaagag tttgagaata
     3361 cggcactaag ctctcagatt gcgaatcaca tgatcgaaaa tcaaatcagt gagaccgaag
     3421 tacccatggg agtgggactg catctgaccg tggacgagac ggattattta gtgccaatgg
     3481 ccaccgaaga accctctgta attgctgcat tgagcaatgg cgctaaaatc gcgcaaggct
     3541 ttaaaactgt gaaccaacag cgcctgatgc gcgggcaaat tgtattttac gacgttgctg
     3601 acccggaatc cctcattgat aaactgcagg ttcgtgaagc cgaggtgttt cagcaagccg
     3661 agctgagtta tccctccatc gttaagcgtg gaggaggttt gcgggactta cagtatcgga
     3721 cttttgatga aagctttgtg tccgttgatt ttttggtgga tgttaaggat gctatggggg
     3781 caaatattgt caacgccatg ttagaaggag ttgccgaact cttccgcgaa tggtttgctg
     3841 agcagaagat tctgtttagt attctgagca attatgccac ggaatccgta gtcaccatga
     3901 aaaccgccat cccagtaagc cgtctatcca aaggatccaa tggtcgggaa attgcagaaa
     3961 agattgtcct ggctagtcgt tatgcgagct tggatccata tcgggctgtt actcacaaca
     4021 aaggaattat gaatggtatc gaagccgtgg ttctggccac gggcaatgat acacgcgccg
     4081 tgtccgctag ttgtcatgct tttgctgtaa aagaggggcg gtaccaagga ttgacctcct
     4141 ggaccctgga tggggaacaa ttgattgggg aaatttctgt gccattagct ctcgccacgg
     4201 taggtggtgc gacaaaggtg ttgccgaaaa gtcaagccgc cgccgatctg ttagccgtga
     4261 ccgatgcaaa agaattatct cgcgtggtgg cggccgtcgg tttggcgcaa aatctggctg
     4321 ctctgcgtgc gttagttagc gaaggcattc aaaagggcca tatggcgtta caagcgcgta
     4381 gtttagccat gactgtcggc gccaccggta aagaagtgga agccgttgcc cagcagttga
     4441 aacgccaaaa aaccatgaat caggatcgcg ctatggccat cttgaatgat ctacgtaagc
     4501 agtagggaat taggaggtaa tatatgaaaa attgtgttat tgtctccgcc gttcgcacag
     4561 ctattggaag ctttaacggg tctttggctt ccacttccgc cattgacctg ggcgctactg
     4621 ttatcaaggc tgctattgaa cgggctaaaa tcgacagtca acacgtggac gaagtgatca
     4681 tgggtaatgt cttgcaggcc ggcttagggc aaaatcccgc ccgtcaagct ctcctgaaaa
     4741 gtggactagc ggaaactgtt tgtggattta cagtaaataa agtgtgcggg tccggtttga
     4801 aaagcgttgc cttggccgct caggcgattc aggccggaca ggcccagtct attgtagctg
     4861 ggggcatgga aaatatgagt ctcgcccctt acctgttgga tgctaaagcg cgtagtggct
     4921 accggttagg ggacgggcaa gtgtacgacg tgattctacg cgatggatta atgtgcgcca
     4981 cccatggtta ccacatgggt atcacagctg agaatgtggc aaaagaatac ggcattaccc
     5041 gcgaaatgca ggatgaatta gccctgcata gtcagcgcaa ggcggccgcc gcaatcgaaa
     5101 gtggagcgtt tacagctgaa attgttcccg taaatgtcgt cacccgtaag aagacctttg
     5161 tgttttccca ggatgaattt ccgaaggcca attctactgc agaggcttta ggtgccttac
     5221 gtccagcgtt tgataaagcc gggactgtta ctgctggtaa cgcatccggg attaatgatg
     5281 gcgctgcggc gctcgtgatc atggaagaga gtgccgcgct agccgctggt ttgactccat
     5341 tggcccgcat taaaagttac gcctctggtg gggttccgcc agccttgatg gggatgggtc
     5401 cagtaccggc cacccagaaa gccttgcaac tagcgggctt acaactagcc gatattgatc
     5461 tcatcgaagc gaacgaagcc ttcgccgctc agtttctcgc tgtaggtaaa aacctagggt
     5521 ttgatagtga aaaagtaaac gtaaacggcg gcgctattgc cctcgggcac ccgattggag
     5581 ccagcggcgc gcggatcttg gtgaccctgc tgcatgccat gcaggctcgg gataaaaccc
     5641 tggggctcgc caccctctgc attggtggcg gacagggtat tgccatggtt attgaacggt
     5701 tgaattaagg aattaggagg ggcgcgccac gctcgagaac tcataaagtc aagtaggaga
     5761 ttaattcaat gagtcacatc cagagagaaa ctagttgttc ccgacctcgt ttgaatagca
     5821 atatggatgc agatctgtac ggatataaat gggcgcgaga taacgtaggc caatctgggg
     5881 ccactattta tcggttatat ggcaaaccag atgctcccga actgtttctc aaacatggca
     5941 aagggtctgt ggccaatgat gttaccgatg aaatggtgcg gttgaactgg ttgacagaat
     6001 ttatgcccct cccgaccatc aaacatttta tcaggactcc agacgatgca tggctattaa
     6061 ctacggccat tcctgggaaa actgcctttc aggtgttgga agaatatccc gattctggtg
     6121 agaatatcgt cgatgcgtta gcggtttttc taagacgtct acatagcatt cccgtttgca
     6181 attgtccctt taattcggac cgggtgttcc gcttggcgca ggctcagtcc cggatgaata
     6241 acggtttggt agatgcctcg gactttgatg atgaacggaa cggctggccc gttgaacagg
     6301 tttggaaaga gatgcataag ctgctgccct tctcccccga cagcgttgtt actcatggag
     6361 atttttctct cgataatctg attttcgacg aaggcaagct aattggctgt atcgatgtgg
     6421 gacgggtagg gattgcggac cggtatcaag acctagcaat tttgtggaac tgcctaggtg
     6481 aattttcccc cagcctacaa aaacggctgt ttcaaaaata cggaatcgat aatcccgaca
     6541 tgaacaaatt acaatttcat ctgatgctag atgagttctt ttagctcgag ccttggtgta
     6601 atgccaactg aataatctgc aaattgcact ctccttcaat ggggggtgct ttttgcttga
     6661 ctgagtaatc ttctgattgc tgatcttgat tgccatcgat cgccggggag tccggggcag
     6721 ttaccattag agagtctaga gaattaatcc atcttcgata gaggaattat gggggaagaa
     6781 cctgtgccgg cggataaagc attaggcaag aaattcaaga aaaaaaatgc ctcctggagc
     6841 attgaagaaa gcgaagctct gtaccgggtt gaggcctggg gggcacctta ttttgccatt
     6901 aatgccgctg gtaacataac cgtctctccc aacggcgatc ggggcggttc gttagatttg
     6961 ttggaactgg tggaagccct gcggcaaaga aagctcggct tacccctatt aattcgtttt
     7021 tccgatattt tggccgatcg cctagagcga ttgaatagtt gttttgccaa ggcgatcgcc
     7081 cgttacaatt ggcgcgccgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9241 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9301 taaaaatagg cgtatcacga ggccctttcg tc
//
